FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_47.3520000005c477.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_47.3520000005c477.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104494
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA3520.33686144659023487No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3270.3129366279403602No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2690.25743104867265104No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC2360.22585028805481655No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1780.1703447087871074No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1590.15216184661320267No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1510.1445059046452428No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.1425919191532528No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1470.14067793366126286No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1400.13397898443929795No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1380.13206499894730797No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA1300.1244090569793481No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC1260.12058108599536815No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1200.11483912951939824No Hit
GCTCCAGTCTGCTAACGTACTCTGCGTTGATAC1080.10335521656745841No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTT307.044227E-527.02973727
TTCGTTG257.729998E-427.02973627
GTATCAT307.090414E-527.0038341
TCTACGC200.00858929526.99093
CCTCCTC200.00858929526.99094
CTCAGAG257.79528E-426.99094
ATCATCG257.79528E-426.99093
CTACGCA200.00858929526.99094
CTGTCTC200.00858929526.99094
TCATCGC257.79528E-426.99094
ACGCTGA451.6710528E-624.0034071
CGCTGAG401.8375944E-523.6170372
GTAGAGT401.8375944E-523.6170374
CGTAGAG401.8375944E-523.6170373
TATCATC352.0264302E-423.1350572
TCGCAGA300.002255363622.4924167
ATCGCAG300.002255363622.4924166
CATCGCA300.002255363622.4924165
ACGTAGA454.5632147E-520.9929222
TAGAGTG454.5632147E-520.9929225
Produced by FastQC (version 0.11.2)