FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_44.3520000005c40d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_44.3520000005c40d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90754
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA3830.42201996606210196No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2700.2975075478766776No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2470.2721643123168125No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA2390.26334927386120727No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC2320.25563611521255264No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA1990.2192740815831809No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA1940.2137646825484276No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA1400.15426317297309208No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA1360.14985565374528945No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1270.13993873548273356No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA1260.13883685567578288No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA1230.13553121625493092No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.13442933644798025No Hit
GTATCAACGCAGAGTGAATGGGCTAAACGTACT1180.1300218172201776No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.1201048989576217No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.10798422108116447No Hit
GGGCAGAGTGGCAAAAAAAAAAAAAAAAAAAAA950.10467858166031249No Hit
GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.10357670185336183No Hit
GAGTGAATGGGCAGAGTGGCAAAAAAAAAAAAA910.10027106243250986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC554.783942E-1026.9867781
TTACCAG200.00858987426.9867745
ACGCATA200.00858987426.9867741
TGCCCAG200.00858987426.9867745
CCCCTTC200.00858987426.9867741
CAACTCA1000.024.28811
GTATCAT501.5383739E-724.28811
TATCATC501.5383739E-724.28812
AGTTGCT401.8363253E-523.61342813
ATCTACG553.8590406E-722.0800912
ACGCTGA707.792551E-921.2038961
GTTGCTA454.5601224E-520.98971614
CTGAGTG658.326424E-820.7590584
TGAGTGA658.326424E-820.7590585
TCTACGC608.9096284E-720.2400843
CTACGCA608.9096284E-720.2400844
CGCTGAG751.7238563E-819.7903042
GCTGAGT701.8191713E-719.2762683
TACGCAG651.9185827E-618.6831535
ATCATCG651.9185827E-618.6831533
Produced by FastQC (version 0.11.2)