FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_43.3520000005c3ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_43.3520000005c3ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91052
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC9731.0686201291569652No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5590.613934894346088No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA4030.442604226156482No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3030.3327768747528885No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA2930.3217941396125291No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA2450.2690770109388042No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG1840.20208232658261213No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT1800.19768923252646836No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA1630.17901858278785748No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1630.17901858278785748No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA1600.17572376224574968No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA1550.17023239467557No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA1520.1669375741334622No Hit
GTGAATGGGACATCCAGCTAAAAAAAAAAAAAA1450.15924965953521064No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1430.15705311250713877No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1400.15375829196503096No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA1380.1515617449369591No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1380.1515617449369591No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1370.15046347142292316No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA1320.14497210385274348No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA1240.13618591574045602No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1230.13508764222642006No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT1170.12849800114220444No Hit
CATCCAGCTAGAAAAAAAAAAAAAAAAAAAAAA930.10213943680534202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGC200.00859417426.9840371
GTATCAT200.00859417426.9840371
CTCCGAC200.00859417426.9840374
GCCCCCG200.00859417426.9840371
ATCTACG352.0270982E-423.1291792
TCTACGC352.0270982E-423.1291793
TACGCAG352.0270982E-423.1291795
TATCTAC352.0270982E-423.1291791
CTACGCA352.0270982E-423.1291794
AGGGAAT300.00225645522.48677
TGCCCAC300.00225645522.48675
CCCCGCA300.00225645522.48673
TATCATC300.00225645522.48672
CCCCCGC300.00225645522.48672
AACGTAG350.00551335719.2743151
TACCACT450.001087701618.02904927
CCCACAC450.001103933817.989364
TGAATAG450.001103933817.989362
TCTCACA450.001103933817.989365
ATACCAC1750.016.22614327
Produced by FastQC (version 0.11.2)