FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_40.3520000005c385.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_40.3520000005c385.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56842
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA7581.3335209880018297No Hit
GTCAAGCTCAACACGTACTCTGCGTTGATACCA5300.932409134090989No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA3650.6421308187607755No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2620.4609267794940361No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA2130.3747229161535484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.2832412652616023No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAAAA1510.2656486400900742No Hit
TATCAACGCAGAGTGAATGGGTCAAGCTCAACA1380.2427782273670877No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA1280.2251856021955596No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1270.2234263396784068No Hit
GTGAATGGGTCAAGCTCAACACGTACTCAAAAA1250.21990781464410117No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1200.21111150205833715No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1190.20935223954118434No Hit
GTCAAGCTCAACACGTACTCAAAAAAAAAAAAA1160.2040744519897259No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1150.2023151894725731No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1140.2005559269554203No Hit
ACGCAGAGTGAATGGGTCAAGCTCAACACGTAC1060.18648182681819783No Hit
GCTCAACACGTACAAAAAAAAAAAAAAAAAAAA1020.17944477674958656No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA990.17416698919812815No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA980.17240772668097534No Hit
GTGAATGGGTCAAGCTAAAAAAAAAAAAAAAAA980.17240772668097534No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA950.1671299391295169No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC930.1636114140952113No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA900.15833362654375285No Hit
CAACACGTACTCAAAAAAAAAAAAAAAAAAAAA870.1530558389922944No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA830.1460187889236832No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC820.14425952640653036No Hit
GTGAATGGGTCAAGCTCAACACAAAAAAAAAAA820.14425952640653036No Hit
AGCTCAACACGTACTCAAAAAAAAAAAAAAAAA740.1301854262693079No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA730.1284261637521551No Hit
GGTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAA710.12490763871784948No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA690.12138911368354385No Hit
GTGAATGGGGTCAAGCTCAAAAAAAAAAAAAAA680.11962985116639104No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.11962985116639104No Hit
GAGTGAATGGGGTCAAGCTCAAAAAAAAAAAAA660.11611132613208544No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.1125928010977798No Hit
GTATCAACGCAGAGTGAATGGGTCAAGCTCAAC630.110833538580627No Hit
GCTCAACACGTACTAAAAAAAAAAAAAAAAAAA600.10555575102916857No Hit
AACGCAGAGTGAATGGGTCAAGCTCAACACGTA590.10379648851201577No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTAAA300.002243468822.4911821
GATACCA1002.474917E-816.22226127
TATCAAC3950.015.0320811
TGATACC1151.3757744E-714.10631526
GTACTCA1755.456968E-1213.88027410
TACTCAA1850.013.85943111
TCAACGC4050.013.6613113
GAGAATG600.00745466313.4947098
CAACGCA4250.013.3359474
ATCAACG4150.013.3321232
ACTCAAA2100.012.85210412
TGGGAAG956.889037E-512.7844616
CAGAGAG750.002212695312.595064
GCAGAGA750.002212695312.595063
ATGGGAA1255.5346554E-611.8753445
TCTGCGT7650.011.83977727
GAGAGAA800.003589517711.807876
TGGGGTC1153.1107877E-511.73452956
GGGAAGC959.924689E-411.3639657
AACGCAG5050.010.95615
Produced by FastQC (version 0.11.2)