FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_4.3520000005bf05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_4.3520000005bf05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77724
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA9801.2608718027893573No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA5870.755236477793217No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA5790.7449436467500385No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA5780.7436570428696413No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA3460.445164942617467No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA3360.4322989038134939No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA3020.3885543718799856No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA2150.2766198342854202No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA2120.27276002264422833No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA1900.2444547372754876No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA1850.23802171787350113No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA1830.2354485101127065No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA1780.22901549071071997No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA1740.22386907518913077No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA1680.21614945190674695No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1660.21357624414595233No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.20842982862436313No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA1610.20714322474396585No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA1480.1904173742988009No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA1310.16854510833204672No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA1300.16725850445164944No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA1280.1646852966908548No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA1260.1621120889300602No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA1180.1518192578868818No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA1140.14667284236529257No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA1110.14281303072410068No Hit
GTATCGACGTCTACAAAAAAAAAAAAAAAAAAA1100.14152642684370334No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA1090.14023982296330606No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC1060.13638001132211416No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA1040.13380680356131952No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA1010.12994699192012762No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA1010.12994699192012762No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1000.12866038803973034No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA990.12737378415933304No Hit
GTGAATGGGTATCGACGTCTAAAAAAAAAAAAA980.1260871802789357No Hit
GTGAATGGGTATCGACGTCTACGTACTCAAAAA950.1222273686377438No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA880.11322114147496269No Hit
GTGAATGGGAGTATCGACGTCAAAAAAAAAAAA870.11193453759456538No Hit
ACGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA870.11193453759456538No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA870.11193453759456538No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA860.11064793371416808No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT840.10807472595337347No Hit
TCTACGTACTCAAAAAAAAAAAAAAAAAAAAAA810.10421491431218156No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA800.10292831043178426No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA800.10292831043178426No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT800.026.9845351
TATCATC900.023.9862562
GAATGGA757.2577677E-1021.587633
TCATCGC1000.021.587634
TCGCAGA1000.021.5737297
ATCGCAG1000.021.5737296
CATCGCA1000.021.5737295
TAGAGTG658.351344E-820.743975
ATCATCG1050.020.5596473
GATACCA1500.019.85261727
AACGTAG701.8122591E-719.274671
CGTAGAG701.8122591E-719.274673
TGGGGGA350.005526228319.2622576
TGATACC1650.018.8681926
GTAGAGT753.7429345E-717.989694
CCTGTAT552.1340385E-417.1609217
TATCAAC5350.015.8880911
GTATCTA604.1228934E-415.7409791
TTGATAC2100.014.825006525
CGTCTAA751.3610095E-414.39175216
Produced by FastQC (version 0.11.2)