FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_36.3520000005c30e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_36.3520000005c30e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107374
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC5180.4824259131633356No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.28591651610259466No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA2810.2617020880287593No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2790.25983943971538737No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA2790.25983943971538737No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.16205040326335984No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1480.13783597518952445No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1420.1322480302494086No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1410.13131670609272264No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.12666008530929276No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.11548419542906105No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1170.10896492633225921No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1130.1052396297055153No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1090.10151433307877139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTC257.6881796E-427.05563423
TCTTCGT257.6881796E-427.05563425
ATCTACG604.5474735E-1126.9925382
CTTCAAC200.00860733426.9799544
CTATACT200.00860733426.9799545
TTACCAC200.00860733426.9799544
TCTACGC604.5474735E-1126.9799543
TACGCAG604.5474735E-1126.9799545
GCCACAC200.00860733426.9799544
CCCCTGA257.8151765E-426.9799543
TATCTAC651.2187229E-1024.9161871
CCCTGAC401.8439818E-523.607464
GTATCAT752.910383E-1123.3935341
ATCGCAG752.910383E-1123.3826266
CTACGCA703.110472E-1023.1256734
CTTCGTT300.002224539622.54636226
TACTCTT300.002224539622.54636222
CAACTCA900.022.493781
CTGAGTG300.002261086122.4832954
TGAGTGA300.002261086122.4832955
Produced by FastQC (version 0.11.2)