FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_32.3520000005c286.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_32.3520000005c286.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80128
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA13021.624900159744409No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC10881.3578274760383386No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA8311.0370906549520766No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA6740.8411541533546327No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA6180.7712659744408945No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA4790.5977935303514377No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA4650.5803214856230032No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA4560.5690894568690096No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA4390.5478734025559105No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT4070.5079373003194888No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA4050.5054412939297125No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA4040.5041932907348242No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA3950.4929612619808307No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA3650.45552116613418525No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3200.3993610223642172No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA3150.3931210063897764No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA3120.3893769968051118No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC3080.3843849840255591No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA2750.3432008785942492No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA2740.34195287539936103No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA2660.3319688498402556No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA2620.32697683706070285No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA2280.2845447284345048No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC2270.28329672523961663No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2190.2733126996805112No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA2190.2733126996805112No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA2190.2733126996805112No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.27206469648562304No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA2000.24960063897763576No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA1940.2421126198083067No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1920.23961661341853036No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA1910.2383686102236422No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA1900.23712060702875398No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA1780.22214456869009583No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1760.21964856230031948No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG1740.21715255591054314No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA1730.21590455271565495No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA1620.20217651757188496No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC1600.1996805111821086No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1560.1946884984025559No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA1550.19344049520766773No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1530.1909444888178914No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC1510.18844848242811502No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA1480.18470447284345048No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA1440.17971246006389777No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA1390.17347244408945686No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA1340.16723242811501599No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA1310.16348841853035143No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA1270.1584964057507987No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA1240.15475239616613418No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC1230.15350439297124602No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA1140.1422723642172524No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA1130.14102436102236424No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA1110.13852835463258786No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA1090.13603234824281152No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA1060.13228833865814696No Hit
CTAACTATCCACGTACTCAAAAAAAAAAAAAAA1050.1310403354632588No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA1040.1297923322683706No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1020.12729632587859424No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA1020.12729632587859424No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA970.12105630990415335No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA970.12105630990415335No Hit
GAATGGGGCTAACTATCCACGTACTCAAAAAAA970.12105630990415335No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA940.11731230031948882No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC940.11731230031948882No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA920.11481629392971246No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA910.11356829073482429No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.11356829073482429No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA900.11232028753993611No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA860.1073282747603834No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA830.10358426517571885No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.10358426517571885No Hit
GTGAATGGGCTAACTATCAAAAAAAAAAAAAAA820.10233626198083068No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA820.10233626198083068No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA405.9340346E-727.01441
GTATCAT900.025.5136011
AACTCAG501.5276783E-724.2977472
ACTCAGA451.6753911E-623.9827653
TCAGAGT451.6753911E-623.9827655
TCTACGC850.023.8064233
CTACGCA850.023.8064234
TACGCAG801.8189894E-1223.6080365
TATCATC1000.022.9478722
GTGTATG300.002239394222.5120031
CTGAGTG553.8556027E-722.0750454
ATCTACG1000.021.5979982
CTCAGAG504.183814E-621.5844924
TATCTAC951.8189894E-1221.327161
ACGCTGA707.665221E-921.2256031
AACGTAG404.953537E-420.2608011
GCTGAGT608.901843E-720.235463
TGAGTGA608.901843E-720.235465
CGCTGAG751.708213E-819.7981642
CCACTTA350.00549180319.28392818
Produced by FastQC (version 0.11.2)