FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_30.3520000005c243.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_30.3520000005c243.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80730
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA4980.6168710516536603No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3530.4372600024773938No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA2850.3530286138981791No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA2740.3394029480986003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2110.2613650439737396No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA2080.25764895330112725No Hit
CACTACGTACTCAAAAAAAAAAAAAAAAAAAAA1870.23163631859284034No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1740.21553325901151987No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1740.21553325901151987No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA1710.21181716833890749No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1710.21181716833890749No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1620.2006688963210702No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1550.1919980180849746No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1450.17961104917626655No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1440.17837235228539577No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1410.17465626161278336No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA1300.1610305958132045No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA1270.1573145051405921No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA1260.1560758082497213No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC1230.1523597175771089No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA1190.14740493001362567No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1150.14245014245014245No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1060.1313018704323052No Hit
GTGAATGGGGTTCGTCACACAAAAAAAAAAAAA1010.1251083859779512No Hit
GTTCGTCACACTACGTACTCAAAAAAAAAAAAA1010.1251083859779512No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA970.12015359841446797No Hit
ACACTACGTACTCAAAAAAAAAAAAAAAAAAAA940.11643750774185557No Hit
GTGAATGGGGTTCGTCACACTACGTACTCAAAA910.11272141706924316No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA850.10528923572401833No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACT830.10281184194227673No Hit
GTGAATGGGTTCGTCACACTACGTACAAAAAAA810.1003344481605351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG200.0084632627.0653427
TACTCTT200.0084632627.0653422
CGCTGAG200.00859262926.981392
ACGCTGA200.00859262926.981391
CAACTCA604.3655746E-1126.981391
GCTGAGT200.00859262926.981393
CGCAAAG200.00859262926.981392
ATCATCG352.0256818E-423.1269073
ATCGCAG352.0256818E-423.1269076
TCATCGC352.0256818E-423.1269074
TATCTAC300.00225554622.4844911
TCGCAGA300.00225554622.4844917
CATCGCA404.995108E-420.2360445
AACTCAG751.720582E-819.7863542
TCAGAGT803.6085112E-818.5497065
ACTCAGA857.2204784E-817.4585483
CTCAGAG857.2204784E-817.4585484
TATCAAC3550.016.3408411
GTATCAT500.002233555316.1888331
TATCATC500.002233555316.1888332
Produced by FastQC (version 0.11.2)