FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_29.3520000005c229.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_29.3520000005c229.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134383
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTACGTACTCAAAAAAAAAAAAAAAAAAAA14951.1124919074585327No Hit
GCTTGGACCTTACGTACTCTGCGTTGATACCAC13350.9934292283994256No Hit
TGCTTGGACCTTACGTACTCTGCGTTGATACCA12960.9644077003787681No Hit
TATCAACGCAGAGTGAATGGGCCTTACGTACTC6730.5008073937923695No Hit
GGCCTTACGTACTCAAAAAAAAAAAAAAAAAAA6240.46434444833051797No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA5720.42564907763630816No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCAAAAA5230.3891861321744566No Hit
GACCTTACGTACTCAAAAAAAAAAAAAAAAAAA5120.38100057298914297No Hit
GTGAATGGGCCTTACGTACTCAAAAAAAAAAAA5000.37207087205970996No Hit
CCTTACGTACTCAAAAAAAAAAAAAAAAAAAAA4510.3356079265978583No Hit
GTGAATGGGACCTTACGTACTCAAAAAAAAAAA4380.3259340839243059No Hit
GAGTACGTAAGGTCCAAAAAAAAAAAAAAAAAA4340.3229575169478282No Hit
GAATGGGACCTTACGTACTCAAAAAAAAAAAAA4130.3073305403213204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4020.2991449811360068No Hit
GTATCAACGCAGAGTGAATGGGCCTTACGTACT3640.2708675948594688No Hit
ACCTTACGTACTCAAAAAAAAAAAAAAAAAAAA3550.2641703191623941No Hit
GTACGTAAGGTCCAAAAAAAAAAAAAAAAAAAA3510.2611937521859164No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA3130.23291636590937842No Hit
TATCAACGCAGAGTGAATGGGACCTTACGTACT3070.2284515154446619No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.22547494846818422No Hit
ACGCAGAGTGAATGGGACCTTACGTACTCAAAA3020.22473080672406479No Hit
GAGTGAATGGGCCTTACGTACTCAAAAAAAAAA2670.19868584567988512No Hit
AACGCAGAGTGAATGGGCCTTACGTACTCAAAA2450.18231472730925788No Hit
GAATGGGCCTTACGTACTCAAAAAAAAAAAAAA2410.1793381603327802No Hit
ACGCAGAGTGAATGGGCCTTACGTACTCTGCGT2380.17710573510042193No Hit
GATACCACTGCTTGGACCTTACGTACTCTGCGT2320.1726408846357054No Hit
GGACCTTACGTACTCAAAAAAAAAAAAAAAAAA2220.1651994671945112No Hit
GCCTTACGTACTCTGCGTAAAAAAAAAAAAAAA1910.1421310731268092No Hit
GACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.14138693138268976No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.13766622266209266No Hit
TACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA1830.13617793917385385No Hit
GCTTGGACCAAAAAAAAAAAAAAAAAAAAAAAA1710.1272482382444208No Hit
GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.12650409650030137No Hit
GTGAATGGGAGGTCCAAAAAAAAAAAAAAAAAA1640.12203924603558486No Hit
GTATCATCGCAGAGTGAATGGGCCTTACGTACT1620.12055096254734601No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA1550.11534197033851007No Hit
GAGTGAATGGGACCTTACGTACTCAAAAAAAAA1530.11385368685027124No Hit
AATGGGACCTTACGTACTCAAAAAAAAAAAAAA1530.11385368685027124No Hit
CCTTACGTACTCTGCGTAAAAAAAAAAAAAAAA1510.11236540336203239No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1480.11013297812967414No Hit
GCCTTACGTACTCTGCGAAAAAAAAAAAAAAAA1450.10790055289731587No Hit
TGCTTGGACCAAAAAAAAAAAAAAAAAAAAAAA1450.10790055289731587No Hit
GACCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.10566812766495762No Hit
AACGCAGAGTGAATGGGACCTTACGTACTCAAA1420.10566812766495762No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT700.026.9746551
GTATTAA200.008623159526.9746551
TTAACGC200.008623159526.9746554
TTTTGGA200.00863885726.9646052
ATTAACG200.00863885726.9646053
TATCATC700.026.9646052
TATTAAC200.00863885726.9646052
TATCTAC800.025.288741
CTGAGTG950.022.71554
ACGCTGA950.022.71551
CTACGCA950.022.71554
CAACTCA603.6199708E-822.478881
TCTACGC900.022.4705053
TTGTACC300.002271529822.47050313
TACGCAG857.2759576E-1222.2144225
TGAGTGA1000.021.5797255
CGCTGAG1000.021.5716842
ATCTACG951.8189894E-1221.2878482
ATCGCAG851.5825208E-1020.6276786
TCATCGC851.5825208E-1020.6276784
Produced by FastQC (version 0.11.2)