FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_28.3520000005c20f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_28.3520000005c20f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78203
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA6030.7710701635487129No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT3760.48079996931064023No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2770.3542063603698068No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA2060.2634170044627444No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.25446594120430166No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1620.2071531782668184No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1530.1956446683631063No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA1450.1854148817820288No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1350.17262764855568202No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1350.17262764855568202No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1320.16879147858777796No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC1290.16495530861987392No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA1230.15728296868406583No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1220.15600424536143115No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA1170.14961062874825773No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA1080.13810211884454562No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA980.12531488561819878No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA910.11636382235975602No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA880.11252765239185199No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA840.10741275910131326No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT840.10741275910131326No Hit
CCCCCATCTCGTCCTCTACGTACTCTGCGTTGA840.10741275910131326No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA830.10613403577867857No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA790.10101914248813984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA200.00856013327.0012821
CATCCAT200.00858696326.9839844
TACCACT751.8189894E-1225.24975627
GTATCAT601.2969394E-924.7511771
TCATCGC551.412991E-824.5308954
TCGCAGA501.5341357E-724.2855857
ATCATCG501.5341357E-724.2855853
ACGCTGA300.002245260222.501071
CTGAGTG300.002253649522.4866564
CATCGCA603.5579433E-822.4866565
ATCGCAG553.8485268E-722.0778056
ATGGGTA701.8129867E-719.2742735
CTACGCA350.005506596519.2742734
TGAGTGA350.005506596519.2742735
ATACCAC1006.366463E-1118.93731526
TGGGTAC501.0237657E-418.8887886
GCGTTGA6700.016.75689927
TATCATC952.5576264E-715.6223072
TCTATCT707.5622745E-515.4194186
GAGTACG1304.292633E-813.5006411
Produced by FastQC (version 0.11.2)