FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_27.3520000005c1e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_27.3520000005c1e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66444
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2520.37926675094816686No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.23929925952681957No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA1500.2257540184215279No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA1320.19866353621094454No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1200.18060321473722232No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1170.17608813436879178No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1150.17307808078983808No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA1130.17006802721088435No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1100.16555294684245378No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1040.15652278610559267No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1010.15200770573716213No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA940.14147251821082416No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA920.13846246463187045No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA870.13093733068448618No Hit
GTGAATGGGGTCGTTGGAAAAAAAAAAAAAAAA840.12642225031605564No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA830.12491722352657877No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA810.12190716994762507No Hit
GGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.11739208957919452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA200.00857400626.9864251
GTATCAT505.0877134E-926.9864251
TATCATC551.4033503E-824.5331132
CAACTCA454.536759E-520.9894431
TCATCGC658.2425686E-820.7587874
TCGCAGA658.308598E-820.7431457
ATCGCAG658.308598E-820.7431456
CATCGCA701.8153696E-719.2614925
CTACGCA501.0200948E-418.8904974
TACGCAG501.026028E-418.876265
ATCATCG753.7197788E-717.990953
ATCTACG552.1165198E-417.173182
TCTACGC552.1165198E-417.173183
TATCTAC552.1165198E-417.173181
CTCAGAG552.1165198E-417.173184
TCAGAGT552.1287777E-417.1602385
TGCGTTG4400.015.99477427
ACTCAGA604.1093864E-415.7420823
TATCAAC4950.015.2650481
AACTCAG657.5461617E-414.5311522
Produced by FastQC (version 0.11.2)