FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_26.3520000005c1ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_26.3520000005c1ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91712
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13161.434926727145848No Hit
GACACAGACAGTACGTACTCTGCGTTGATACCA12501.3629623168178646No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA8970.9780617585484995No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA8550.9322662247034195No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA7600.8286810886252617No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT7490.8166870202372645No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA7400.8068736915561758No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.7501744591765527No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA5820.6345952547103978No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA5810.6335048848569435No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA5100.5560886252616888No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA5000.5451849267271458No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA4340.47322051639916257No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA4180.4557745987438939No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA3930.42851535240753663No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA3380.3685450104675506No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA3180.34673761339846476No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA3130.34128576413119327No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA3100.3380146545708304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3030.3303820655966504No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA3030.3303820655966504No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA2960.32274947662247033No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA2940.32056873691556176No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA2910.3172976273551989No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA2760.3009420795533845No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA2610.2845865317515701No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA2550.2780443126308444No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA2490.27150209351011867No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.2627791346824843No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA2380.25950802512212146No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.25514654570830425No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.2529658060013957No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT2180.2377006280530356No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA2180.2377006280530356No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA2140.2333391486392184No Hit
GTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2130.23224877878576414No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA2080.22679692951849267No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA2070.2257065596650384No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA1940.2115317515701326No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1930.21044138171667828No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA1930.21044138171667828No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA1900.20717027215631542No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA1850.20171842288904396No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1840.20062805303558967No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGT1810.1973569434752268No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.19408583391486392No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1760.19190509420795532No Hit
AGACAGTACGTACTCAAAAAAAAAAAAAAAAAA1730.18863398464759246No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA1680.183182135380321No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.17991102581995813No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC1590.17336880669923238No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA1580.1722784368457781No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA1530.16682658757850663No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA1510.16464584787159806No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA1490.16246510816468948No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA1470.16028436845778088No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA1450.1581036287508723No Hit
GTGAATGGGACACAGACAGTACGTACTCAAAAA1410.15374214933705513No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA1390.15156140963014655No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA1340.1461095603628751No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1330.1450191905094208No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA1300.14174808094905791No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA1280.13956734124214934No Hit
GACACAGACAGTACGTACAAAAAAAAAAAAAAA1240.13520586182833216No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC1240.13520586182833216No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA1240.13520586182833216No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA1210.1319347522679693No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA1170.12757327285415213No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA1160.12648290300069784No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA1140.12430216329378926No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA1140.12430216329378926No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA1110.12103105373342637No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGTAC1090.1188503140265178No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA1080.1177599441730635No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC1050.11448883461270064No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA1050.11448883461270064No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA1040.11339846475924634No Hit
AACGCAGAGTGAATGGGACACAGACAGTACGTA1040.11339846475924634No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA1020.11121772505233775No Hit
GAATGGGACACAGACAGTACAAAAAAAAAAAAA1020.11121772505233775No Hit
AACGCAGAGTGAATGGGACACAGACAGTAAAAA1020.11121772505233775No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA1020.11121772505233775No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC990.10794661549197487No Hit
GAGTGAATGGGGACACAGACAGAAAAAAAAAAA960.10467550593161201No Hit
GAGTGAATGGGACACAGACAGTACAAAAAAAAA950.10358513607815771No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA940.10249476622470341No Hit
ATGGGACACAGACAGAAAAAAAAAAAAAAAAAA940.10249476622470341No Hit
AACGCAGAGTGAATGGGACACAGACAGTACAAA920.10031402651779484No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT1350.026.9770871
GTATTAA257.8112923E-426.9770871
ATTAACG257.8112923E-426.9770873
TATTAAC257.8112923E-426.9770872
TTAACGC257.8112923E-426.9770874
CTACGCA1300.025.9395084
ATCTACG1250.025.8980052
TCTACGC1250.025.8980053
TACGCAG1250.025.8980055
TATCTAC1250.025.8980051
TATCATC1400.025.0501522
CAACTCA1550.023.496171
TATGGGA352.0308315E-423.1232174
TAACGCA300.002259987622.4809065
TCGCAGA1550.021.7557137
ATCGCAG1600.021.0758486
GTATGGG454.5733977E-520.9821783
GTGTATG454.5733977E-520.9821781
ATCATCG1550.020.8854853
TCATCGC1550.020.8854854
Produced by FastQC (version 0.11.2)