FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_25.3520000005c1a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_25.3520000005c1a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24251
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1480.6102841119953817No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA1350.5566780751309225No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA1140.47008370788833453No Hit
CAACGAACCAAGACGTACTCTGCGTTGATACCA840.34637746897035177No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA810.33400684507855344No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA770.3175126798894891No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA770.3175126798894891No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA750.3092655972949569No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA750.3092655972949569No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAA750.3092655972949569No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAG690.28452434951136035No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA670.2762772669168282No Hit
GTGAATGGGACAACGAACCAAAAAAAAAAAAAA660.2721537256195621No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA600.2474124778359655No Hit
TATCAACGCAGAGTGAATGGGACAACGAACCAA590.24328893653869943No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA580.23916539524143332No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA560.23091831264690116No Hit
AACCAAGACGAAAAAAAAAAAAAAAAAAAAAAA530.2185476887551029No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT530.2185476887551029No Hit
GTATCAACGCAGAGTGAATGGGCAACGAACCAA510.21030060616057067No Hit
CAACGAACCAAAAAAAAAAAAAAAAAAAAAAAA500.20617706486330462No Hit
GAGTGAATGGGCAACGAACCAAAAAAAAAAAAA470.19380644097150632No Hit
AACGCAGAGTGAATGGGCAACGAACCAAAAAAA460.18968289967424024No Hit
TATCAACGCAGAGTGAATGGGCAACGAACAAAA450.18555935837697415No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA440.18143581707970804No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.17318873448517588No Hit
GTGAATGGGCAACGAACCAAGACAAAAAAAAAA400.1649416518906437No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA390.16081811059337758No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACGTA380.1566945692961115No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA370.15257102799884542No Hit
AACCAAGACGTACTAAAAAAAAAAAAAAAAAAA360.1484474867015793No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA340.14020040410704712No Hit
AACCAAGACGTACAAAAAAAAAAAAAAAAAAAA340.14020040410704712No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT320.13195332151251496No Hit
GTGAATGGGCCAACGAACCAAAAAAAAAAAAAA320.13195332151251496No Hit
ATCAACGCAGAGTGAATGGGCAACGAACCAAGA310.12782978021524885No Hit
AACGCAGAGTGAATGGGCAACGAACAAAAAAAA310.12782978021524885No Hit
TATCAACGCAGAGTGAATGGGCCAACGAACCAA310.12782978021524885No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA310.12782978021524885No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA310.12782978021524885No Hit
CAACGAACCAAGACAAAAAAAAAAAAAAAAAAA300.12370623891798276No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA290.11958269762071666No Hit
TATCAACGCAGAGTGAATGGGGCAACGAACCAA290.11958269762071666No Hit
GTCTTGGTTCGTTGAAAAAAAAAAAAAAAAAAA290.11958269762071666No Hit
GTGAATGGGCAACGAAAAAAAAAAAAAAAAAAA290.11958269762071666No Hit
GTGAATGGGGCAACGAACCAAAAAAAAAAAAAA290.11958269762071666No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA280.11545915632345058No Hit
GAATGGGACAACGAACCAAAAAAAAAAAAAAAA280.11545915632345058No Hit
GTGAATGGGACAACGAACAAAAAAAAAAAAAAA280.11545915632345058No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.11545915632345058No Hit
GAGTGAATGGGCAACGAACAAAAAAAAAAAAAA270.11133561502618448No Hit
AACCAAGACGTAAAAAAAAAAAAAAAAAAAAAA270.11133561502618448No Hit
ACGCAGAGTGAATGGGACAACGAACCAAAAAAA270.11133561502618448No Hit
GAGTGAATGGGCCAACGAACCAAAAAAAAAAAA260.10721207372891839No Hit
ACCAAGACGTACTCAAAAAAAAAAAAAAAAAAA250.10308853243165231No Hit
GAATGGGCCAACGAACCAAAAAAAAAAAAAAAA250.10308853243165231No Hit
GCAACGAACCAAGACGTACTCTGCGTTGATACC250.10308853243165231No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTT300.002202767222.49828110
TGGGGTC300.002202767222.4982818
GGGGTCT300.002202767222.4982819
GCGTTGA952.7284841E-1119.89321727
TGGGACA657.2809955E-414.5373516
TGCGTTG1406.7848305E-913.4989726
AACAACG600.00727843713.49896912
TCTGCGT3900.013.15284227
ATGGGAC853.7436918E-412.7049125
ATGGGGA750.002144798612.5990385
TATCAAC2400.011.8360021
ATGGGGG800.003480411111.8115975
TCAACGC2705.456968E-1210.4991983
AATGGGA2851.8189894E-1210.4202574
CTGCGTT1904.732592E-79.94660925
CTCTGCG5200.09.86463126
CAACGCA3005.0931703E-119.4492794
GTATCAA2203.3302967E-79.2228581
ACTCTGC5600.09.16001525
ATCAACG3109.640644E-119.1444642
Produced by FastQC (version 0.11.2)