FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_24.3520000005c187.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_24.3520000005c187.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60083
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC6261.0418920493317578No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC4920.8188672336601035No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC2110.3511808664680525No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC2060.3428590449877669No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2010.3345372235074813No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA2000.33287285921142423No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC1920.31955794484296723No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA1910.3178935805469101No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC1850.3079073947705674No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC1760.2929281161060533No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC1750.2912637518099962No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA1630.27129138025731075No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA1490.24799028011251104No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC1490.24799028011251104No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.23633973004011116No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC1280.2130386298953115No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA1080.17975134397416906No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1040.1730938867899406No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT980.16310770101359784No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA980.16310770101359784No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA930.15478587953331224No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA880.14646405805302665No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA850.1414709651648553No Hit
GTACGTGTGTAGTGTGAAAAAAAAAAAAAAAAA850.1414709651648553No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA810.1348135079806268No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC810.1348135079806268No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA770.1281560507963983No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT760.1264916865003412No Hit
ACACTACACACGTACTCAAAAAAAAAAAAAAAA750.12482732220428408No Hit
ACACTACACAAAAAAAAAAAAAAAAAAAAAAAA740.12316295790822694No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC740.12316295790822694No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC700.11650550072399848No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC700.11650550072399848No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC690.11484113642794135No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA690.11484113642794135No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA680.11317677213188422No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA680.11317677213188422No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC660.10984804353976999No Hit
CCCTCCACACTACACACGTACTCTGCGTTGATA640.10651931494765575No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGAG200.00856212526.9900111
TCGCAGA257.757998E-426.990017
ATCATCG257.757998E-426.990013
ATCGCAG257.757998E-426.990016
TCATCGC257.757998E-426.990014
GGGGAGG200.00856212526.990017
GTATCAT307.0657436E-526.9900071
ATACCAC950.022.7663427
TATCATC404.96399E-420.2425082
TGGGTGT608.7965964E-720.2425066
GATACCA1100.019.6618426
CATCGCA350.00548500719.2785765
TATCAAC1950.018.6853921
TGGGGAG651.8944193E-618.685396
ATGGGTG753.7000973E-717.993345
ATCAACG2200.016.562052
TCAACGC2350.016.0791553
TTGATAC4050.016.02075827
GTTGATA4350.014.6051326
CAACGCA2700.014.4946334
Produced by FastQC (version 0.11.2)