FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_23.3520000005c17a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_23.3520000005c17a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86970
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA15171.7442796366563182No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA9821.1291249856272279No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA7450.8566172243302288No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG5690.6542485914683224No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA5630.6473496608025756No Hit
GGATAGCAGATACGTACTCTGCGTTGATACCAC4520.5197194434862596No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.45302978038404046No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA3750.4311831666091756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.4185351270553065No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA3450.39668851328044147No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA3120.35874439461883406No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC3100.35644475106358514No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA3080.3541451075083362No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA2920.3357479590663447No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC2800.32195009773485106No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA2800.32195009773485106No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2580.2966540186271128No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT2290.26330918707600326No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA2200.252960791077383No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC2130.24491203863401173No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA2080.2391629297458894No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1880.2161664941934No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA1870.21501667241577557No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1860.21386685063815108No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG1840.21156720708290214No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT1760.2023686328619064No Hit
TATCAACGCAGAGTGAATGGGTAGCAGATACGT1730.19891916752903302No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT1550.17822237553179257No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.16672415775554789No Hit
GTGAATGGGATAGCAGATACGTAAAAAAAAAAA1440.16557433597792343No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA1410.16212487064505002No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC1390.15982522708980107No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1350.1552259399793032No Hit
GAATGGGATAGCAGATACGTACTCAAAAAAAAA1300.14947683109118087No Hit
CAGATACGTACTCAAAAAAAAAAAAAAAAAAAA1230.1414280786478096No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.14027825687018514No Hit
GATACGTACTCTAAAAAAAAAAAAAAAAAAAAA1140.13107968264918937No Hit
GTGAATGGGATAGCAGAAAAAAAAAAAAAAAAA1120.12878003909394042No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC1120.12878003909394042No Hit
GGATAGCAGAAAAAAAAAAAAAAAAAAAAAAAA1120.12878003909394042No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTA1100.1264803955386915No Hit
GGATAGCAGATACGTACTAAAAAAAAAAAAAAA1090.12533057376106704No Hit
GCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.1195814648729447No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACA1010.11613199954007129No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.1126825342071979No Hit
GTGAATGGGTAGCAGATACGTACTCAAAAAAAA940.1080832470967No Hit
GAATGGGATAGCAGATACGTACAAAAAAAAAAA930.10693342531907554No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA920.10578360354145107No Hit
GTGAATGGGGATAGCAGATACGTACTCAAAAAA890.10233413820857767No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGCT200.00849176827.04904724
TATTAAC307.0796144E-527.0023042
GTATTAA307.107467E-526.9867611
CAGCCCG200.008587901526.986764
GTATCTA200.008587901526.986761
GTATCAT1000.026.986761
ATTAACG257.7916373E-426.986763
CCGACCG200.008587901526.986764
TATCATC1050.025.716482
CAACTCA1400.025.0591321
ATCATCG1100.024.5334173
ACTCAGA1550.023.5045953
TCATCGC1150.023.4667474
AACTCAG1500.023.4019972
CTCAGAG1500.023.3885234
TCAGAGT1450.023.2644485
CTGAGTG703.074092E-1023.1315064
TGAGTGA703.074092E-1023.1315065
AACGTAG653.3069227E-922.8349511
ACGCTGA900.022.4889641
Produced by FastQC (version 0.11.2)