FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_22.3520000005c151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_22.3520000005c151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88591
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGTAGTTCTGACACAAAAAAAAAAAA9071.0238060299578964No Hit
TATCAACGCAGAGTGAATGGGTAGTTCTGACAC8000.9030262667765349No Hit
GTTCTGACACGTACAAAAAAAAAAAAAAAAAAA7340.8285265997674707No Hit
GTTCTGACACGTACTCAAAAAAAAAAAAAAAAA5420.6118002957411024No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACAAAAA4700.5305279317312143No Hit
GTATCAACGCAGAGTGAATGGGTAGTTCTGACA3740.42216477971803No Hit
GTGAATGGGGTAGTTCTGACACAAAAAAAAAAA3640.41087695138332336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3330.3758846835457326No Hit
CGGTAGTTCTGACACGTACTCTGCGTTGATACC3180.3589529410436726No Hit
TATCAACGCAGAGTGAATGGGGTAGTTCTGACA3020.34089241570814194No Hit
GGGTAGTTCTGACACGTACTCTGCGTTGATACC3000.3386348500412006No Hit
GTGAATGGGGTAGTTCAAAAAAAAAAAAAAAAA2680.30251379937013917No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.30251379937013917No Hit
GTGAATGGGTAGTTCTGACACGAAAAAAAAAAA2610.2946123195358445No Hit
GTGAATGGGTAGTTCTGACACGTACAAAAAAAA2600.29348353670237387No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACAAAA2560.28896840536849117No Hit
GGGTAGTTCTGACACAAAAAAAAAAAAAAAAAA2550.2878396225350205No Hit
GTTCTGACACAAAAAAAAAAAAAAAAAAAAAAA2420.2731654456999018No Hit
GTGAATGGGGTAGTTCTGACACGTACTCAAAAA2290.2584912688647831No Hit
GTGAATGGGTAGTTCTGACACGTACTCAAAAAA2160.2438170920296644No Hit
GTATCATCGCAGAGTGAATGGGTAGTTCTGACA2090.23591561219536974No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACT2030.22914291519454572No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.22801413236107504No Hit
GTTCTGACACGTAAAAAAAAAAAAAAAAAAAAA1990.22462778386066304No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1970.2223702181937217No Hit
GAGTGAATGGGTAGTTCTGACACAAAAAAAAAA1880.2122111726924857No Hit
GTGAATGGGGTAGTTCTGACACGTACAAAAAAA1860.2099536070255444No Hit
GTGAATGGGTAGTTCAAAAAAAAAAAAAAAAAA1820.20543847569166168No Hit
AACGCAGAGTGAATGGGTAGTTCTGACACGTAC1750.197536995857367No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGTACA1710.19302186452348433No Hit
TATCTACGCAGAGTGAATGGGTAGTTCTGACAC1700.19189308169001365No Hit
GACACGTACTCAAAAAAAAAAAAAAAAAAAAAA1620.1828628190222483No Hit
GTTCTGACACGAAAAAAAAAAAAAAAAAAAAAA1610.18173403618877765No Hit
GTGAATGGGTAGTTCTGACAAAAAAAAAAAAAA1570.17721890485489497No Hit
TTCTGACACAAAAAAAAAAAAAAAAAAAAAAAA1540.17383255635448297No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACG1520.17157499068754162No Hit
GTATCAACGCAGAGTGAATGGGGTAGTTCTGAC1450.16367351085324694No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACGTAC1430.16141594518630561No Hit
GTGAATGGGTAGTTCTGACACGTAAAAAAAAAA1410.15915837951936426No Hit
ACGCAGAGTGAATGGGTAGTTCTGACACGAAAA1400.15802959668589361No Hit
GAATGGGTAGTTCTGACACAAAAAAAAAAAAAA1300.14674176835118694No Hit
CAACTCAGAGTGAATGGGTAGTTCTGACACAAA1270.1433554198507749No Hit
GGTATCAACGCAGAGTGAATGGGTAGTTCTGAC1260.14222663701730423No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1250.14109785418383358No Hit
ACGCAGAGTGAATGGGGTAGTTCTGACACAAAA1190.13432515718300955No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1140.1286812430156562No Hit
ATCAACGCAGAGTGAATGGGTAGTTCTGACACA1130.12755246018218555No Hit
GTGAATGGGTAGTTCTGAAAAAAAAAAAAAAAA1130.12755246018218555No Hit
GGGTAGTTCTGACACGTACTCAAAAAAAAAAAA1080.1219085460148322No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.11739341468094953No Hit
ACGCAGAGTGAATGGGGTAGTTCAAAAAAAAAA1010.11400706618053752No Hit
AGGTAGTTCTGACACGTACTCTGCGTTGATACC1010.11400706618053752No Hit
GGGTAGTTCTGACACGTACAAAAAAAAAAAAAA1000.11287828334706686No Hit
GAGTGAATGGGGTAGTTCTGACACAAAAAAAAA960.1083631520131842No Hit
GAATGGGGTAGTTCAAAAAAAAAAAAAAAAAAA950.10723436917971352No Hit
GACACGTACAAAAAAAAAAAAAAAAAAAAAAAA940.10610558634624286No Hit
GTTCTGACACGTACTAAAAAAAAAAAAAAAAAA940.10610558634624286No Hit
CAACGCAGAGTGAATGGGTAGTTCTGACACAAA930.1049768035127722No Hit
ATCAACGCAGAGTGAATGGGGTAGTTCTGACAC920.1038480206793015No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA890.1004616721788895No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG257.776217E-426.996614
ACGCTGA257.776217E-426.996611
CGGTGGT257.776217E-426.996615
TGAGTGA257.776217E-426.996615
TCTACAC200.00857349526.9966093
TATTAAC200.00857349526.9966092
CCGGTGG200.00857349526.9966094
GTATCAT900.025.49681
CAACTCA1150.024.6490781
TCAGAGT1050.024.4255055
TATCTAC1550.024.3840351
TATCATC950.024.1548612
AACTCAG1200.023.6220342
CTCAGAG1200.023.6220344
TTCAGAC352.0201366E-423.1399542
ATCTACG1600.022.7783892
TCTACGC1600.022.7783893
ACTCAGA1250.022.6771533
GTTCAGA300.00224989922.4971751
GCTGAGT300.00224989922.4971753
Produced by FastQC (version 0.11.2)