FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_21.3520000005c137.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_21.3520000005c137.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105273
Sequences flagged as poor quality0
Sequence length33
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2970.28212362144139524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2710.2574259306754818No Hit
GTTCAAGGTAAAAAAAAAAAAAAAAAAAAAAAA2470.2346280622761772No Hit
GTTCAAGGTACGTACTCAAAAAAAAAAAAAAAA2340.2222792168932205No Hit
GGTACGTACTCTGCGTTGATACAAAAAAAAAAA2110.20043125967722017No Hit
GTTCAAGGAAAAAAAAAAAAAAAAAAAAAAAAA1740.1652845458949588No Hit
GTGAATGGGTACGTACTCTGCGTTGATACAAAA1710.16243481234504573No Hit
CCCCAGTTCAAGGTACGTACTCTGCGTTGATAC1700.16148490116174138No Hit
GTACGTACTCTGCGTTGATACAAAAAAAAAAAA1650.15673534524521957No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA1460.1386870327624367No Hit
TATCAACGCAGAGTGAATGGGTACGTACTCTGC1400.13298756566261055No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA1290.12253854264626257No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1290.12253854264626257No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1160.11018969726330587No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1160.11018969726330587No Hit
TACGTACTCTGCGTTGATACAAAAAAAAAAAAA1140.10828987489669717No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1130.10733996371339279No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1120.10639005253008844No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1110.10544014134678409No Hit
ACGCAGAGTGAATGGGTACGTACTCTGCGTTGA1080.10259040779687098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT604.5474735E-1126.992381
CGGGCCA257.814812E-426.9795345
TCAGCCA200.00860719926.9795345
TACGCAG505.175025E-926.9795345
GCTGCCC200.00860719926.9795344
CGCAGTT200.00860719926.9795348
TATCATC604.5474735E-1126.9795342
TATTCCA200.00860719926.9795345
CTACGCA554.820322E-1026.9795344
CGCCGCC200.00860719926.9795345
CAACTCA850.025.4045941
ATCTACG551.427361E-824.526852
TCTACGC551.427361E-824.526853
CTCAGAG850.023.8054714
CAGCCCC401.8437364E-523.6070924
TCAGAGT801.8189894E-1223.6070925
TGTATGG401.8437364E-523.6070922
GTGTATG352.0257852E-423.1363261
AACTCAG1000.022.9326042
ACTCAGA950.022.7196083
Produced by FastQC (version 0.11.2)