FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_2.3520000005bec0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_2.3520000005bec0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65901
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2810.426397171514848No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA1910.2898286824175657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1780.2701021228812916No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA1530.23216643146537988No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1480.22457929318219755No Hit
GTGGTAGTGAGCTAAAAAAAAAAAAAAAAAAAA1370.2078875889591964No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1360.2063701613025599No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1300.19726559536274108No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1260.1911958847361952No Hit
GCTCACTACCACAAAAAAAAAAAAAAAAAAAAA1210.18360874645301284No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1150.17450418051319405No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1150.17450418051319405No Hit
GTGAATGGGGTGGTAGTGAGCTAAAAAAAAAAA1060.16084733160346582No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1050.15932990394682933No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA1030.1562950486335564No Hit
ACGTGGTAGTGAGCTAAAAAAAAAAAAAAAAAA920.13960334441055522No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA840.12746392315746347No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.11987678487428112No Hit
CCTTAGCTCACTACCACGTACTCTGCGTTGATA770.11684192956100817No Hit
GCCTAGCTCACTACCACGTACTCTGCGTTGATA770.11684192956100817No Hit
CTCTAGCTCACTACCACGTACTCTGCGTTGATA670.10166765299464348No Hit
TAGCTCACTACCACGTACTCTGCGTTGATACCA660.10015022533800699No Hit
CCCCTAGCTCACTACCACGTACTCTGCGTTGAT660.10015022533800699No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTC307.004118E-527.0304223
TCTTCGT257.6983153E-427.03041825
TACTCTT351.9954577E-423.16892822
TTCGTTG300.002227526622.52534927
CTCTTCG300.002227526622.52534924
CTTCGTT300.002227526622.52534926
GTTGATA2600.020.27281427
TATCAAC3000.014.866731
ATCAACG3050.014.6008072
TCAACGC3150.014.1372893
CGTTGAT3850.013.69073126
CTAGCAA600.00747161513.49468513
AATGGGT1101.330036E-613.4946844
ATTAGCT1152.1921405E-612.907967
ATGGGTA853.910328E-412.700885
CAACGCA3700.012.0358014
TGGGGTG1051.7620642E-411.5668736
ATGGGAG959.962243E-411.3639455
GGGGTGG959.962243E-411.3639457
TCTGCGT16150.010.7954327
Produced by FastQC (version 0.11.2)