FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_16.3520000005c088.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_16.3520000005c088.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80789
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA9261.1461956454467812No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA6740.8342719924742229No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC4730.5854757454603968No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA4570.5656710690811868No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA4360.5396774313334736No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA3310.4097092425949077No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.4022824889527039No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA3150.3899045662156977No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA3070.3800022280260927No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC2750.3403928752676726No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA2630.32553936798326505No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC2530.3131614452462588No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC2490.30821027615145624No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC2200.27231430021413805No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.26612533884563494No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC2150.26612533884563494No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA2140.2648875465719343No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA2090.25869858520343114No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC1910.23641832427681986No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA1900.23518053200311922No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA1880.232704947455718No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA1800.22280260926611298No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA1790.22156481699241232No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC1790.22156481699241232No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA1630.2017601406132023No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA1570.19433338697099853No Hit
GTGAATGGGTGTTCACGTACTCAAAAAAAAAAA1550.1918578024235973No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA1540.19062001014989663No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC1440.17824208741289035No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA1420.17576650286548912No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC1380.17081533377068658No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA1350.1671019569495847No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC1330.16462637240218347No Hit
GCTATGGTGTTCACGTACTCAAAAAAAAAAAAA1310.1621507878547822No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC1310.1621507878547822No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.16091299558108157No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1280.1584374110336803No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC1270.1571996187599797No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA1260.15596182648627907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.1472972805703747No Hit
GTGTTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.13739494238076966No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA1110.13739494238076966No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA1100.13615715010706902No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA1070.13244377328596715No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA1070.13244377328596715No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTTCA1010.12501701964376338No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC990.12254143509636212No Hit
GGGCTATGGTGTTCACGTACTCAAAAAAAAAAA960.11882805827526023No Hit
GTGAATGGGGCTATGGAAAAAAAAAAAAAAAAA960.11882805827526023No Hit
GTGAATGGGGCTATGGTAAAAAAAAAAAAAAAA920.11387688918045773No Hit
GTGAATGGGCTATGGTGTTCACGTACTCAAAAA900.11140130463305649No Hit
AAGCTATGGTGTTCACGTACTCTGCGTTGATAC900.11140130463305649No Hit
CAACTCAGAGTGAATGGGCTATGGTGAAAAAAA890.11016351235935584No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC890.11016351235935584No Hit
GAATGGGCTATGGTGTAAAAAAAAAAAAAAAAA870.1076879278119546No Hit
TATCTACGCAGAGTGAATGGGCTATGGTGAAAA850.10521234326455334No Hit
GTATCATCGCAGAGTGAATGGGCTATGGTGAAA820.10149896644345145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA700.026.985121
GTATCAT604.3655746E-1126.985121
ATTAACG200.00858686626.985123
TTAACGC200.00858686626.985124
TATCATC651.2187229E-1024.9093422
GCGGCTA300.002253746422.48766
GTTCACT300.002253746422.487616
ACTTACT300.002253746422.487620
CTTACTC300.002253746422.487621
CTACTGC300.002253746422.48764
ATCTACG658.302777E-820.7577862
TATCTAC658.302777E-820.7577861
ATCATCG658.302777E-820.7577863
CTACGCA608.889565E-720.238844
TTACTCT350.00542823919.32300422
TTCACTT350.005506816319.27508517
TCTACGC701.81406E-719.2750853
CACTTAC350.005506816319.27508519
TACTGCT350.005506816319.2750855
TCACTTA350.005506816319.27508518
Produced by FastQC (version 0.11.2)