FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_12.3520000005c001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_12.3520000005c001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86967
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA7830.9003415088481838No Hit
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7660.8807938643393471No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA7080.8141019007209631No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA6970.8014534248623041No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA6370.7324617383605275No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA6340.7290121540354387No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC4600.5289362631802867No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4310.49559028137109473No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA4180.48064208262904323No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA3580.4116503961272667No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA3440.3955523359435188No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA3320.3817539986431635No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA2950.3392091253004013No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA2610.300113836282728No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA2530.29091494474915774No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA2530.29091494474915774No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA2480.28516563754067636No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA2340.2690675773569285No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2220.25526924005657314No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA2080.23917117987282532No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1990.22882242689755886No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT1950.2242229811307737No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA1940.22307311968907748No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA1750.20122575229684822No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1700.19547644508836684No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA1680.1931767222049743No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA1610.18512769211310037No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA1610.18512769211310037No Hit
GATTCACGTACTCTGCGAAAAAAAAAAAAAAAA1560.179378384904619No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA1560.179378384904619No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA1540.17707866202122644No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG1480.1701794933710488No Hit
GGATTCACGTACTCTGCGAAAAAAAAAAAAAAA1460.16787977048765623No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGT1420.16328032472087115No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.16328032472087115No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA1420.16328032472087115No Hit
GTGAATGGGCGTAAGGATTCACGTACTCAAAAA1400.1609806018374786No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA1350.1552312946289972No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA1350.1552312946289972No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA1240.14258281877033818No Hit
ACGCAGAGTGAATGGGCGTAAGGATTCACGTAC1190.1368335115618568No Hit
GGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.1356836501201605No Hit
GTGAATGGGATTCACGTACTCTGCGTAAAAAAA1170.13453378867846424No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC1160.13338392723676798No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA1160.13338392723676798No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA1120.12878448146998286No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.12418503570319776No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.12303517426150148No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA1060.12188531281980522No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA1040.11958558993641266No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA1020.11728586705302012No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1000.11498614416962756No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA990.1138362827279313No Hit
ATCAACGCAGAGTGAATGGGATTCACGTACTCT940.10808697551944992No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA940.10808697551944992No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA940.10808697551944992No Hit
GATTCACGTACTCTGCGTAAAAAAAAAAAAAAA940.10808697551944992No Hit
ATTCACGTACTCTGCGTAAAAAAAAAAAAAAAA940.10808697551944992No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA910.10463739119436109No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA900.1034875297526648No Hit
GGATTCACGTACAAAAAAAAAAAAAAAAAAAAA890.10233766831096852No Hit
GAGTGAATGGGATTCACGTACTCTGCGTAAAAA890.10233766831096852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA750.026.9826981
GTATCAT750.026.9826981
GTATTAA200.00859412526.9826981
TATCTAC950.024.1424141
TATCATC801.8189894E-1223.6098612
ATCTACG1000.022.9352932
TACGCAG1000.022.9352935
CTACGCA1000.022.9352934
ACGCTGA950.022.7222731
CGCTGAG900.022.4855822
CTGAGTG900.022.4855824
GCTGAGT900.022.4855823
TGAGTGA900.022.4855825
TCTACGC1050.021.8431363
AACTCAG951.8189894E-1221.3021322
ATCGCGT404.997678E-420.2370246
ACTCAGA953.092282E-1119.8819893
CTCAGAG953.092282E-1119.8819894
TCAGAGT903.3287506E-1019.4875055
ATCATCG907.052222E-917.9884663
Produced by FastQC (version 0.11.2)