FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate2_1.3520000005beb3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate2_1.3520000005beb3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111519
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA28072.5170598732054628No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA25422.2794322043777293No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA13871.243734251562514No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA13271.1899317605071782No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA11361.018660497314359No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9680.8680135223594185No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA9270.8312484868049391No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA8920.7998637003559931No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA8760.7855163694079036No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA7850.7039159246406442No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA7530.675221262744465No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA7130.6393529353742411No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA6980.6259023126104072No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.6187286471363624No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA6200.5559590742384706No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA5440.4878092522350452No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA5220.46808167218142205No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA4760.42683309570566447No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA4480.40172526654650775No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA4480.40172526654650775No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT4300.385584519229907No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT4300.385584519229907No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT4260.3819976864928846No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC3950.35419973278096106No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA3760.3371622772801047No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA3730.334472152727338No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA3670.32909190362180435No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA3670.32909190362180435No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA3640.32640177906903756No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA3530.316537989042226No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.30846761538392564No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA3390.30398407446264764No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA3390.30398407446264764No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA3380.303087366278392No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA3360.30129394990988084No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA3170.2842564944090245No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA3160.2833597862247689No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA2920.26183878980263453No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA2780.24928487522305615No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA2740.24569804248603375No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA2720.2439046261175226No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA2600.23314412790645542No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC2590.2322474197221998No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA2520.22597046243241062No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA2510.225073754248155No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA2480.22238362969538825No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.20982971511580983No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.20803629874729868No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.19996592508899827No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2150.1927922596149535No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA2150.1927922596149535No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA2030.18203176140388633No Hit
CACACGTACTCTGAAAAAAAAAAAAAAAAAAAA1880.16858113864005236No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA1810.16230418135026317No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.1614074731660076No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC1800.1614074731660076No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA1780.1596140567974964No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA1740.156027224060474No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA1700.1524403913234516No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1690.151543683139196No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG1680.15064697495494042No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA1580.14167989311238444No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA1570.14078318492812883No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA1530.13719635219110643No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCT1530.13719635219110643No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA1530.13719635219110643No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA1520.13629964400685085No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA1480.13271281126982845No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG1440.12912597853280608No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA1440.12912597853280608No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA1440.12912597853280608No Hit
ACGCTGAGTGAATGGGCAGACACACGTACTCAA1430.12822927034855047No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA1410.12643585398003926No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA1410.12643585398003926No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA1370.12284902124301687No Hit
GCAGACACACGTACTCAAAAAAAAAAAAAAAAA1330.11926218850599449No Hit
AACGTAGAGTGAATGGGCAGACACACGTACTCA1260.11298523121620531No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA1230.1102951066634385No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.10939839847918292No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTA1210.10850169029492732No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.10670827392641612No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGA1120.10043131663662694No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCTA257.805163E-426.9869671
TGTATGG257.805163E-426.9869672
GTATCAT1600.026.9869651
GGTTTCA200.00859778826.9869651
TATTAAC356.5365257E-626.9869652
TAACGCA356.559425E-626.9748445
ATTAACG356.559425E-626.9748443
TTAACGC356.559425E-626.9748444
GTATGGG257.8257016E-426.9748423
TATCATC1700.024.6057642
GTATTAA451.681834E-623.9884131
CAACTCA1950.023.52711
TATCTAC1950.023.52711
TTACTCT351.9866743E-423.20468922
TAATGGG300.002258200222.4891416
TTAATGG300.002258200222.4891415
TATGGGC300.002264110422.4790364
ATCTACG2100.021.8465922
TCTACGC2100.021.8367773
CTACGCA2100.021.8367774
Produced by FastQC (version 0.11.2)