FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_95.3520000005be63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_95.3520000005be63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28149
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGTTAGCAAAAAAAAAAAAAAAAAAAAA1310.4653806529539238No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1300.4618281288855732No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1270.45117055668052153No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1050.3730150271768091No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA870.30906959394649897No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA840.2984120217414473No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA800.28420192546804507No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA780.2770968773313439No Hit
GTGAATGGGGAGTACGTTAGCAAAAAAAAAAAA740.26288678105794167No Hit
CCTCCAGTCTGCTAACGTACTCTGCGTTGATAC730.2593342569895911No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA730.2593342569895911No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.21670396816938436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.20959892003268324No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT590.20959892003268324No Hit
GTCCAGTCTGCTAACGTACTCTGCGTTGATACC550.19538882375928096No Hit
GTGAATGGGTCCAGTCAAAAAAAAAAAAAAAAA540.1918362996909304No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA480.170521155280827No Hit
CTCCAGTCTGCTAACGTACTCTGCGTTGATACC480.170521155280827No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.15986358307577533No Hit
CTTCCAGTCTGCTAACGTACTCTGCGTTGATAC430.1527585349390742No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA390.13854843866567196No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA380.1349959145973214No Hit
ACGCAGAGTGAATGGGTCCAGTCTGAAAAAAAA350.12433834239226971No Hit
CCAGTCTGCTAACGTACTCTGCGTTGATACCAC350.12433834239226971No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC340.12078581832391914No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.11723329425556857No Hit
ACTCCAGTCTGCTAACGTACTCTGCGTTGATAC320.11368077018721803No Hit
GAGTGAATGGGATCCAGTCAAAAAAAAAAAAAA310.11012824611886746No Hit
GTACGTTAGCAAAAAAAAAAAAAAAAAAAAAAA300.1065757220505169No Hit
TATCAACGCAGAGTGAATGGGTCCAGTCAAAAA300.1065757220505169No Hit
GTGAATGGGTCCAGTCTGAAAAAAAAAAAAAAA300.1065757220505169No Hit
GTGAATGGGGTCCAGTCAAAAAAAAAAAAAAAA290.10302319798216632No Hit
ACGCAGAGTGAATGGGTCCAGTCAAAAAAAAAA290.10302319798216632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC306.851279E-527.037527
GTTGATA757.015178E-616.222526
TTGATAC757.015178E-616.222527
TGCGTTG2850.013.28157827
TATCAAC2350.012.6332921
TCTGCGT7600.012.62935727
CGTTGAT1001.0568235E-412.16687525
ATCAACG2551.8189894E-1211.1132432
ATTCCAG850.00552363811.1132437
CTCTGCG9150.010.63770526
CTTCCAG900.00844898810.4958418
TCAACGC2655.0931703E-1110.1846443
ACTCTGC9600.010.13906325
CTGCGTT4000.010.13906226
CAACGCA2801.4733814E-109.6390384
TACTCTG10350.09.53496424
ACTCCAG1300.0012161339.3424517
GCGTTGA1453.0731372E-49.32327624
GTACTCT10700.09.22307223
CGTACTC10800.09.13767322
Produced by FastQC (version 0.11.2)