FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_94.3520000005be4a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_94.3520000005be4a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29831
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC1850.6201602359961115No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1580.5296503637155978No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA1280.42908383895947166No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA890.29834735677650764No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA810.2715296168415407No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA710.23800744192283194No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA650.2178941369716067No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT620.2078374844959941No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC610.20448526700412323No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA550.184371962052898No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA510.17096309208541452No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.16425865710167276No Hit
ACGCAGAGTGAATGGGGTACGTTACTGCAAAAA470.15755422211793102No Hit
GTGAATGGGTACGTTACTGCAAAAAAAAAAAAA460.15420200462606012No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA450.15084978713418926No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA450.15084978713418926No Hit
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA440.1474975696423184No Hit
GTGAATGGGAGTACGTTACTGCAAAAAAAAAAA430.14414535215044752No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA380.12738426469109315No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA380.12738426469109315No Hit
CTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1240320471992223No Hit
GTGAATGGGGAGTACGTTACTGCAAAAAAAAAA360.1206798297073514No Hit
CCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.11397539472360967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA608.251991E-720.3030326
ATACCAC651.7777929E-618.74125927
TATCAAC2350.017.2211911
TGCGTTG3100.016.59172427
GTTGATA852.2791754E-514.3315523
TTGATAC852.2791754E-514.3315524
ATCAACG2700.013.989562
TCTGCGT9100.013.83283327
TGATACC903.9059974E-513.53535525
CGTTGAT903.9059974E-513.53535522
GTATCAA1501.8671926E-812.5906051
CAGAGAG750.00217595912.5906054
GCAGAGA750.00217595912.5906053
GAGAGAA750.00217595912.5906056
TCCTCCT800.003530570811.8036917
AGAGAGA800.003530570811.8036915
TCAACGC3100.011.7492973
CTGCGTT4450.011.55827926
CACTCCT1204.6452406E-511.2416117
CAACGCA3300.011.0372174
Produced by FastQC (version 0.11.2)