FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_90.3520000005bdc1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_90.3520000005bdc1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33974
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA1270.3738152705009713No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA1170.34438099723317833No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA1100.32377700594572323No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG880.2590216047565786No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA850.2501913227762407No Hit
ACGCAGAGTGAATGGGCAGTCTCTGGAAAAAAA830.24430446812268203No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.23841761346912343No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC800.23547418614234414No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA790.23253075881556484No Hit
ACGCAGAGTGAATGGGCAGTCTCAAAAAAAAAA700.20603991287455112No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.1913227762406546No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA650.1913227762406546No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA640.1883793489138753No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA610.17954906693353742No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA590.1736622122799788No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA560.1648319302996409No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.16188850297286161No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCAAAAA550.16188850297286161No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA500.14717136633896508No Hit
GTGAATGGGACAGTCTCTGGAAAAAAAAAAAAA500.14717136633896508No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.14128451168540648No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA480.14128451168540648No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA480.14128451168540648No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA470.1383410843586272No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA460.1353976570318479No Hit
AACGCAGAGTGAATGGGCAGTCTCTGGAAAAAA450.1324542297050686No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG440.1295108023782893No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA420.12362394772473069No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA410.12068052039795137No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA410.12068052039795137No Hit
GTCTCTGGAAAAAAAAAAAAAAAAAAAAAAAAA400.11773709307117207No Hit
GAAACGTACTCTGCGAAAAAAAAAAAAAAAAAA390.11479366574439276No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.11479366574439276No Hit
GAGTGAATGGGCAGTCTCTGGAAAAAAAAAAAA390.11479366574439276No Hit
GTGAATGGGGAGTACGTTTCCAAAAAAAAAAAA380.11185023841761346No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA380.11185023841761346No Hit
GTGAATGGGAACGTACTCAAAAAAAAAAAAAAA370.10890681109083417No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA350.10301995643727556No Hit
ACGCAGAGTGAATGGGACAGTCTCTGGAAAAAA350.10301995643727556No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA340.10007652911049626No Hit
GTGAATGGGGCAGTCTCAAAAAAAAAAAAAAAA340.10007652911049626No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT455.3592885E-826.9734521
TATCATC455.3592885E-826.9734522
ACGCTGA200.00853595126.973451
CGCACAG200.00853595126.973455
TGAGTGA200.00853595126.973455
ATCATCG608.61648E-720.230093
TCATCGC608.61648E-720.230094
TCGCAGA651.8559767E-618.6739277
ATCGCAG651.8559767E-618.6739276
CATCGCA651.8559767E-618.6739275
GTATCAA2100.015.4134011
GGTATCA801.3403664E-515.1725651
ATCAGTC954.2507363E-614.1965537
TCCAGTC1001.09367225E-412.1380527
TCTGCGT8700.011.98957727
TATCAAC2900.011.16142751
ATCAACG3150.010.7037512
TCAACGC3100.010.4413373
TCAGTCT2851.8189894E-1210.4108068
TGGAAAC11350.09.2684116
Produced by FastQC (version 0.11.2)