FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_87.3520000005bd64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_87.3520000005bd64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26469
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA1270.4798065661717481No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1230.464694548339567No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1210.45713853942347654No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA950.35891042351429975No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA760.2871283388114398No Hit
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA760.2871283388114398No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA660.2493482942309872No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA660.2493482942309872No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT640.24179228531489666No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT640.24179228531489666No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.23045827194076088No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA590.22290226302467037No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA590.22290226302467037No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC560.21156824965053458No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA540.20401224073444407Illumina Single End PCR Primer 1 (100% over 29bp)
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA540.20401224073444407No Hit
GAGTGAATGGGAGCTGACGAAAAAAAAAAAAAA530.2002342362763988No Hit
GTGAATGGGAGCTGACGAAGAAAAAAAAAAAAA480.1813442139861725No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA480.1813442139861725No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.17756620952812724No Hit
ACGCAGAGTGAATGGGGCTGACGAAAAAAAAAA470.17756620952812724No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA460.173788205070082No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA440.16623219615399146No Hit
GTGAATGGGAGCTGACAAAAAAAAAAAAAAAAA400.15112017832181043No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA400.15112017832181043No Hit
AACGCAGAGTGAATGGGGCTGACGAAAAAAAAA400.15112017832181043No Hit
GAGTGAATGGGGCTGACGAAAAAAAAAAAAAAA380.1435641694057199No Hit
GTGAATGGGAGCTGACGAAGTACAAAAAAAAAA360.13600816048962938No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA350.1322301560315841No Hit
TATCAACGCAGAGTGAATGGGGCTGACGAAAAA340.12845215157353884No Hit
GAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA320.12089614265744833No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA290.10956212928331255No Hit
GAATGGGAGCTGACGAAAAAAAAAAAAAAAAAA290.10956212928331255No Hit
AACGCAGAGTGAATGGGAGCTGACGAAAAAAAA280.10578412482526729No Hit
GAGCTGACGAAGTACAAAAAAAAAAAAAAAAAA280.10578412482526729No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG257.700651E-426.9542874
TATCATC306.98009E-526.9542852
ATCATCG351.9889303E-423.1036723
ATCGCAG351.9889303E-423.1036726
TCATCGC351.9889303E-423.1036724
CATCGCA351.9889303E-423.1036725
TGAGTGA300.00222847522.4619055
TCGCAGA404.905695E-420.2157147
ACGCTGA350.00544615119.253061
GTATCAT450.001083707417.9695241
TATCAAC2300.013.4771431
TGCGTTG1951.8189894E-1213.23239327
ATCAACG2600.011.9220882
TCAACGC2650.011.6971433
TGGGAGC2005.984475E-1011.4555726
CAACGCA2800.011.0705094
ATGGGAG2254.2055035E-910.182735
TCTGCGT4750.010.00676827
CTGCGTT3055.456968E-129.79585326
GGGAGCT2401.2136297E-89.5463097
Produced by FastQC (version 0.11.2)