FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_86.3520000005bd4b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_86.3520000005bd4b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24549
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT2791.1365025051936941No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA2471.0061509633793637No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA2060.8391380504297528No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA2040.8309910790663572No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA2030.8269175933846592No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC1980.80655016497617No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA1850.7535948511140983No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA1820.7413743940690048No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC1630.6639781661167461No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA1430.5825084524827895No Hit
GTGAATGGGTGTTGCCAAAAAAAAAAAAAAAAA1200.48881828180373943No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA1190.48474479612204163No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA1180.4806713104403438No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC1170.476597824758646No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA1070.4358629679416677No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA1030.4195690252148764No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA970.3951281111246894No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA950.3869811397612937No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA940.3829076540795959No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA940.3829076540795959No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA890.3625402256711068No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA880.3584667399894089No Hit
GTGAATGGGGTTGCCAAAAAAAAAAAAAAAAAA850.3462462829443155No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA850.3462462829443155No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA840.3421727972626176No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA830.33809931158091977No Hit
GTGAATGGGGTTGTTGCCAAAAAAAAAAAAAAA830.33809931158091977No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG760.30958491180903497No Hit
GTGAATGGGTTGTTGCCAAAAAAAAAAAAAAAA760.30958491180903497No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA750.30551142612733717No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA730.29736445476394147No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA710.2892174834005458No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA690.2810705120371502No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA660.26885005499205666No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA640.26070308362866107No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC640.26070308362866107No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA590.2403356552201719No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA590.2403356552201719No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC580.23626216953847406No Hit
GTTGTTGCCAAAAAAAAAAAAAAAAAAAAAAAA560.22811519817507844No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA550.22404171249338056No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG510.2077477697665893No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA500.20367428408489147No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA470.19145382703979796No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA470.19145382703979796No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA470.19145382703979796No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA460.18738034135810014No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC450.18330685567640229No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA450.18330685567640229No Hit
GTGAATGGGTTGCCAAAAAAAAAAAAAAAAAAA430.17515988431300664No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT420.1710863986313088No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA420.1710863986313088No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA420.1710863986313088No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA410.167012912949611No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA400.16293942726791316No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA400.16293942726791316No Hit
GTGAATGGGTACGTTGTTGCCAAAAAAAAAAAA380.15479245590451748No Hit
ACGCAGAGTGAATGGGGTTGTTGCCAAAAAAAA380.15479245590451748No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1507189702228197No Hit
TTCGGCAACAACGTACTCTGCGTTGATACCACT370.1507189702228197No Hit
GTGTTCGGCAAAAAAAAAAAAAAAAAAAAAAAA370.1507189702228197No Hit
CAACGTACTCTAAAAAAAAAAAAAAAAAAAAAA360.14664548454112183No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA360.14664548454112183No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.14664548454112183No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA360.14664548454112183No Hit
ACGCAGAGTGAATGGGGTTGCCAAAAAAAAAAA360.14664548454112183No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA360.14664548454112183No Hit
ACGCAGAGTGAATGGGTTGTTGCCAAAAAAAAA360.14664548454112183No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA350.142571998859424No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA350.142571998859424No Hit
GAGTGAATGGGGTTGCCAAAAAAAAAAAAAAAA340.13849851317772618No Hit
ACGTACTCTAAAAAAAAAAAAAAAAAAAAAAAA340.13849851317772618No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA340.13849851317772618No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA330.13442502749602833No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA320.13035154181433053No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA320.13035154181433053No Hit
GAGTGAATGGGGTTGTTGCCAAAAAAAAAAAAA320.13035154181433053No Hit
ATGTTCGGCAACAACGTACTCTGCGTTGATACC320.13035154181433053No Hit
AACGCAGAGTGAATGGGGTTGTTGCCAAAAAAA310.1262780561326327No Hit
TATCTACGCAGAGTGAATGGGCAACAACGTACT300.12220457045093486No Hit
ACGTACTCTGAAAAAAAAAAAAAAAAAAAAAAA290.11813108476923703No Hit
GTACGTTGTTGCCAAAAAAAAAAAAAAAAAAAA290.11813108476923703No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCAA290.11813108476923703No Hit
TCAACGCAGAGTGAATGGGCAACAACGTACTCT270.10998411340584137No Hit
ACGCAGAGTGAATGGGTGTTGCCAAAAAAAAAA260.10591062772414356No Hit
GAGTGAATGGGCAACAACGTACTCTGCGAAAAA250.10183714204244573No Hit
ACGTACTCTGCAAAAAAAAAAAAAAAAAAAAAA250.10183714204244573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00846851926.9877552
TCTACGC200.00846851926.9877553
TACGCAG200.00846851926.9877555
TATCTAC200.00846851926.9877551
CTACGCA200.00846851926.9877554
ATGGGTG559.087413E-619.6274595
TGGGTGT559.087413E-619.6274596
GGGTGTT707.220258E-515.4215757
GCTGTTC751.3003536E-414.3934697
TCTGAAA700.001279777513.49387810
CTGAAAA802.2499014E-413.49387711
AATGGGT1356.3368134E-812.9941044
TATCAAC1305.783895E-712.4558861
GTATCAA1255.18504E-611.8746121
ATCAACG1401.417804E-611.5661812
TCAACGC1452.1638934E-611.1673473
CTCTGAA850.00549188811.1126059
ACTCTGA850.00549188811.1126058
CTGCGAA850.00549188811.11260511
TCTGCGT4300.011.00584927
Produced by FastQC (version 0.11.2)