FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_83.3520000005bcec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_83.3520000005bcec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30791
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC4321.403007372284109No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.8444025851709915No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.48715533759864893No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1330.43194439933746875No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.3897242700789192No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA930.3020363093111623No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA630.20460524179143255No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA610.19810983729011722No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA610.19810983729011722No Hit
GTGAATGGGGAGTCCAAAAAAAAAAAAAAAAAA570.1851190282874866No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA560.18187132603682896No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA510.16563281478354067No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT500.16238511253288299No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC470.15264200578091No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA470.15264200578091No Hit
GTGAATGGGAGTCCTTCAAAAAAAAAAAAAAAA440.14289889902893702No Hit
GAGTACGTCACAAAAAAAAAAAAAAAAAAAAAA430.13965119677827936No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.13315579227696406No Hit
GTGAATGGGAGTCCTAAAAAAAAAAAAAAAAAA390.12666038777564873No Hit
GAGTCCTTCTGTGACGTACTCTGCGTTGATACC330.10717417427170275No Hit
GGCGCAGTCCAAAAAAAAAAAAAAAAAAAAAAA310.10067876977038746No Hit
GTGAATGGGGAGTCCTAAAAAAAAAAAAAAAAA310.10067876977038746No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTAC200.008447641527.0228391
ATCTACG200.00851529126.9788282
TCTACGC200.00851529126.9788283
TACGCAG200.00851529126.9788285
GATACCA350.0053362419.33356726
TATCAAC2550.016.9555071
GCGTTGA2350.016.7009127
ATCAACG2700.014.488632
CGTTGAT852.2866478E-514.32958427
TCAACGC2850.012.7794453
TGGGAGT800.00353578911.8032376
CAACGCA3100.011.7488444
TGCGTTG4050.011.02729327
TCTGCGT7600.010.684340527
AACGCAG3700.010.2082045
CTGCGTT4600.010.00301826
GTATCAA1853.7874834E-69.4945111
ATGGGAG1651.1414081E-48.9929435
CTCTGCG9500.08.6899326
ACGCAGA5700.08.2964861
Produced by FastQC (version 0.11.2)