FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_80.3520000005bc9c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_80.3520000005bc9c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27251
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA3721.3650875197240468No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAC3231.1852776044915783No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA2781.02014604968625No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA2640.968771788191259No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA2300.8440057245605666No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA2300.8440057245605666No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.7192396609298741No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA1620.5944735972991817No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA1560.5724560566584712No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA1390.510073024843125No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA1380.50640343473634No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA1330.4880554842024146No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA1200.4403508128142087No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1150.4220028622802833No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA1100.4036549117463579No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.39264614142600274No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA1060.38897655131921766No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA1050.3853069612124326No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1020.37429819089207733No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC960.35228065025136696No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA920.33760228982422663No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA900.33026310961065647No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA870.3192543392903013No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA830.304575978863161No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.29356720854280577No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT790.2898976184360207No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA770.2825584382224505No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA700.25687130747495507No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA670.24586253715459983No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA660.24219294704781474No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.2348537668342446No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA590.21650581630031926No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA590.21650581630031926No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA580.21283622619353418No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA570.20916663608674912No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA570.20916663608674912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.20549704597996404No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA560.20549704597996404No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG540.19815786576639388No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC530.19448827565960883No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA530.19448827565960883No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA500.1834795053392536No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA490.17980991523246853No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA490.17980991523246853No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA480.17614032512568348No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC450.16513155480532823No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA450.16513155480532823No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA450.16513155480532823No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC440.16146196469854318No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA430.1577923745917581No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA420.15412278448497305No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA400.14678360427140288No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA400.14678360427140288No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.14678360427140288No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA390.1431140141646178No Hit
CTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.1431140141646178No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA390.1431140141646178No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA390.1431140141646178No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA380.13944442405783272No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC370.13577483395104767No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA360.13210524384426262No Hit
AACTATCCACGTACAAAAAAAAAAAAAAAAAAA360.13210524384426262No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT350.12843565373747753No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA340.12476606363069245No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA340.12476606363069245No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA340.12476606363069245No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA340.12476606363069245No Hit
CCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.12109647352390737No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA330.12109647352390737No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC320.1174268834171223No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA320.1174268834171223No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA310.11375729331033725No Hit
AACGCAGAGTGAATGGGCTAACTATAAAAAAAA310.11375729331033725No Hit
CTAACTATCCACAAAAAAAAAAAAAAAAAAAAA310.11375729331033725No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA300.11008770320355217No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA290.10641811309676709No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA280.10274852298998202No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA280.10274852298998202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1950.017.992661
GTATCAA703.6920446E-617.3500651
ATCAACG2200.015.3346552
ACGGCTA550.00411267314.7212697
TCAACGC2400.014.05676653
CAACGCA2550.013.22989754
TCTGCGT4800.012.39269327
TGGGGCT2007.417839E-910.7955966
CTCTGCG5850.010.39946326
CCCGGCT1050.002222749910.281529
GGGGCTA2305.9644663E-99.9741937
TGCGTTG1904.7281537E-79.96159127
ATGGGGC2203.2750904E-89.8141785
ACTCTGC6300.09.65664425
CGGCTAA3251.8189894E-129.549958
CCGGCTA1701.4668207E-59.5255257
AACGCAG3600.09.3711781
AATGGGG3601.8189894E-128.996334
TACTCTG6900.08.81693624
GTACTCT7300.08.33381623
Produced by FastQC (version 0.11.2)