FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_8.3520000005b395.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_8.3520000005b395.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5702
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA1572.753419852683269No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA601.052262364082778No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA520.9119607155384075No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC460.8067344791301297No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA440.7716590669940372No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA330.5787443002455279No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA330.5787443002455279No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA330.5787443002455279No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC310.5436688881094354No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA290.5085934759733427No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA250.4384426517011574No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC230.4033672395650649No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.36829182742897226No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA190.3332164152928797No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA190.3332164152928797No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA150.2630655910206945No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT150.2630655910206945No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA150.2630655910206945No Hit
GGCACTGTACGTACAAAAAAAAAAAAAAAAAAA140.2455278849526482No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA130.22799017888460188No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA120.2104524728165556No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA120.2104524728165556No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA100.175377060680463No Hit
CCCCCCATGAGCACTGTACGTACTCTGCGTTGA100.175377060680463No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA100.175377060680463No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA100.175377060680463No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA100.175377060680463No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA90.1578393546124167No Hit
CATGAGCACTGTACGTACTCTTCGTTGATACCA90.1578393546124167No Hit
ACGCAGAGTGAATGGGCATGAGCACAAAAAAAA90.1578393546124167No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACT90.1578393546124167No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1578393546124167No Hit
GTCATGAGCACTGTACGTACTCTGCGTTGATAC90.1578393546124167No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC80.14030164854437038No Hit
GGCATGAGCACTGTACGTACTCTGCGTTGATAC80.14030164854437038No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA80.14030164854437038No Hit
GGCACTGTACGTACTCAAAAAAAAAAAAAAAAA80.14030164854437038No Hit
GACATGAGCACTGTACGTACTCTGCGTTGATAC70.1227639424763241No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
GTATCAACGCAGAGTGAATGGGCATGAGCAAAA70.1227639424763241No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA70.1227639424763241No Hit
ACGCAGAGTGAATGGGCATGAGCAAAAAAAAAA70.1227639424763241No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA70.1227639424763241No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GAATGGGCACTGTACGTACCTAAAAAAAAAAAA60.1052262364082778No Hit
GCATGAGCACAAAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA60.1052262364082778No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA60.1052262364082778No Hit
CCCCCCCATGAGCACTGTACGTACTCTGCGTTG60.1052262364082778No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA60.1052262364082778No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC60.1052262364082778No Hit
AACGCAGAGTGAATGGGCATGAGCAAAAAAAAA60.1052262364082778No Hit
GCACTGTACGTACCTGAAAAAAAAAAAAAAAAA60.1052262364082778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC702.7484675E-617.3571431
TCAACGC705.7571604E-515.4285723
ATCAACG705.7571604E-515.4285722
TCTGCGT1402.0008883E-1014.46428627
CAACGCA751.0388411E-414.44
AACGCAG853.0137924E-412.7058835
CTCTGCG1703.7343852E-911.91176526
CCATGAG1203.2680888E-511.258
ACTCTGC1808.774805E-911.2525
TACTCTG1851.3198587E-810.94594624
GTACTCT1758.1385224E-810.79999923
GAGCACT1952.8847353E-810.38461612
AGCACTG2004.1967724E-810.12499913
GCAGAGT1256.462352E-49.723
ACGCAGA1309.276324E-49.3461531
CAGAGTG1309.276324E-49.3461534
AGAGTGA1309.276324E-49.3461535
CGCAGAG1309.276324E-49.3461532
TGAGCAC2303.275909E-78.80434811
CGTACTC2201.9169838E-68.59090922
Produced by FastQC (version 0.11.2)