FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_74.3520000005bbc3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_74.3520000005bbc3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25631
Sequences flagged as poor quality0
Sequence length33
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACAGACAGTACGTACTCTGCGTTGATACCA5652.2043619055050523No Hit
GTGAATGGGACACAGACAAAAAAAAAAAAAAAA4011.564511724084117No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3821.4903827396512037No Hit
GTGAATGGGACACAGACAGAAAAAAAAAAAAAA3121.2172759548983652No Hit
GACACAGACAGAAAAAAAAAAAAAAAAAAAAAA2701.0534118840466622No Hit
AGACAGTACGTACTCTGCGTTGATACCACTGCT2591.0104951035855019No Hit
ACGCAGAGTGAATGGGACACAGACAAAAAAAAA2360.9207600171667121No Hit
GACACAGACAGTACAAAAAAAAAAAAAAAAAAA2190.8544340837267371No Hit
ACGCAGAGTGAATGGGACACAGACAGAAAAAAA1980.7725020483008856No Hit
GAGTGAATGGGACACAGACAAAAAAAAAAAAAA1670.6515547579103429No Hit
TATCAACGCAGAGTGAATGGGACACAGACAAAA1570.6125395029456517No Hit
GACACAGACAAAAAAAAAAAAAAAAAAAAAAAA1410.5501150950021458No Hit
AACGCAGAGTGAATGGGACACAGACAAAAAAAA1400.5462135695056767No Hit
GACACAGACAGTAAAAAAAAAAAAAAAAAAAAA1340.522804416526862No Hit
GACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.522804416526862No Hit
GAGTGAATGGGACACAGACAGAAAAAAAAAAAA1270.49549373805157815No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGAA1200.4681830595762943No Hit
GAATGGGACACAGACAAAAAAAAAAAAAAAAAA1170.456478483086887No Hit
TATCAACGCAGAGTGAATGGGACACAGACAGTA1150.4486754320939488No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.4213647536186649No Hit
GTGAATGGGACACAGACAGTAAAAAAAAAAAAA1030.40185712613631924No Hit
GACACAGACAGTACGAAAAAAAAAAAAAAAAAA980.3823494986539737No Hit
AACGCAGAGTGAATGGGACACAGACAGAAAAAA950.37064492216456635No Hit
GTGAATGGGGACACAGACAAAAAAAAAAAAAAA910.3550388201786899No Hit
GTGAATGGGACACAGAAAAAAAAAAAAAAAAAA830.3238266162069369No Hit
GAATGGGACACAGACAGAAAAAAAAAAAAAAAA820.3199250907104678No Hit
GTGAATGGGGACACAGACAGAAAAAAAAAAAAA820.3199250907104678No Hit
CAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.31212203971752955No Hit
ATCAACGCAGAGTGAATGGGACACAGACAAAAA790.3082205142210604No Hit
CAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.2965159377316531No Hit
GACAGTACAAAAAAAAAAAAAAAAAAAAAAAAA750.292614412235184No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA720.2809098357457766No Hit
GTGAATGGGACACAGACAGTACAAAAAAAAAAA660.2575006827669619No Hit
CAACTCAGAGTGAATGGGACACAGACAAAAAAA660.2575006827669619No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAAA650.25359915727049276No Hit
ATGGGACACAGACAAAAAAAAAAAAAAAAAAAA630.24579610627755455No Hit
GAATGGGGACACAGACAAAAAAAAAAAAAAAAA620.2418945807810854No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.23799305528461626No Hit
CAACTCAGAGTGAATGGGACACAGACAGAAAAA610.23799305528461626No Hit
GACAGTACGTACTCAAAAAAAAAAAAAAAAAAA600.23409152978814715No Hit
GACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAA580.2262884787952089No Hit
ACACAGACAGTACGTACTCTGCGTTGATACCAC580.2262884787952089No Hit
GACACAGACAGTACGTACTCAAAAAAAAAAAAA570.2223869532987398No Hit
GAGTGAATGGGGACACAGACAAAAAAAAAAAAA570.2223869532987398No Hit
GTGAATGGGACACAAAAAAAAAAAAAAAAAAAA570.2223869532987398No Hit
ACGCAGAGTGAATGGGACACAGACAGTAAAAAA570.2223869532987398No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.2223869532987398No Hit
ACAGTACGTACTCTGCGTTGATACCACTGCTTC550.21458390230580157No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.21068237680933244No Hit
GGACACAGACAGAAAAAAAAAAAAAAAAAAAAA510.1989778003199251No Hit
GGACACAGACAAAAAAAAAAAAAAAAAAAAAAA490.19117474932698686No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAGA470.1833716983340486No Hit
CAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1794701728375795No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGT450.17556864734111038No Hit
CAACGCAGAGTGAATGGGACACAGACAAAAAAA450.17556864734111038No Hit
TATCAACGCAGAGTGAATGGGACACAGAAAAAA440.17166712184464125No Hit
TATCTACGCAGAGTGAATGGGACACAGACAAAA440.17166712184464125No Hit
ATCAACGCAGAGTGAATGGGACACAGACAGAAA430.16776559634817215No Hit
ACGCAGAGTGAATGGGACACAGAAAAAAAAAAA430.16776559634817215No Hit
CAGACAGAAAAAAAAAAAAAAAAAAAAAAAAAA420.16386407085170301No Hit
GACACAGACAGTACGTAAAAAAAAAAAAAAAAA420.16386407085170301No Hit
GGACACAGACAGTACGTACTCTGCGTTGATACC420.16386407085170301No Hit
AATGGGACACAGACAAAAAAAAAAAAAAAAAAA410.1599625453552339No Hit
GTATCAACGCAGAGTGAATGGGACACAGACAGA400.15606101985876478No Hit
GAATGGGGACACAGACAGAAAAAAAAAAAAAAA400.15606101985876478No Hit
AATGGGACACAGACAGAAAAAAAAAAAAAAAAA400.15606101985876478No Hit
GAATGGGACACAGACAGTAAAAAAAAAAAAAAA400.15606101985876478No Hit
ACGCAGAGTGAATGGGACACAAAAAAAAAAAAA390.15215949436229564No Hit
ATGGGACACAGACAGAAAAAAAAAAAAAAAAAA390.15215949436229564No Hit
GGTATCAACGCAGAGTGAATGGGACACAGACAG390.15215949436229564No Hit
GAGTGAATGGGACACAGACAGTAAAAAAAAAAA380.14825796886582654No Hit
GTATCATCGCAGAGTGAATGGGACACAGACAAA380.14825796886582654No Hit
CAACGCAGAGTGAATGGGACACAGACAGAAAAA370.1443564433693574No Hit
CAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1443564433693574No Hit
GAGTGAATGGGACACAGAAAAAAAAAAAAAAAA370.1443564433693574No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1404549178728883No Hit
GTACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.1404549178728883No Hit
GTGAATGGGGACACAGAAAAAAAAAAAAAAAAA350.13655339237641917No Hit
GAGTGAATGGGGACACAGACAGAAAAAAAAAAA340.13265186687995006No Hit
GGACACAGACAGTACAAAAAAAAAAAAAAAAAA330.12875034138348096No Hit
CAGTACGTACTCAAAAAAAAAAAAAAAAAAAAA330.12875034138348096No Hit
GACACAGACAGTACGTACTCTTCGTTGATACCA330.12875034138348096No Hit
GAGTGAATGGGACACAAAAAAAAAAAAAAAAAA320.12484881588701183No Hit
ACGCAGAGTGAATGGGACACAGACAGTACAAAA310.1209472903905427No Hit
AACGTAGAGTGAATGGGACACAGACAAAAAAAA300.11704576489407358No Hit
AACGCAGAGTGAATGGGACACAGACAGTAAAAA300.11704576489407358No Hit
ACGCAGAGTGAATGGGGACACAGACAAAAAAAA300.11704576489407358No Hit
AACGCAGAGTGAATGGGACACAGACAGTACAAA300.11704576489407358No Hit
AGACAGTACAAAAAAAAAAAAAAAAAAAAAAAA290.11314423939760446No Hit
ACACAGACAAAAAAAAAAAAAAAAAAAAAAAAA290.11314423939760446No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGTA290.11314423939760446No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA280.10924271390113534No Hit
AACGTAGAGTGAATGGGACACAGACAGAAAAAA280.10924271390113534No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGTAC280.10924271390113534No Hit
ACGCAGAGTGAATGGGGACACAGACAGAAAAAA260.1014396629081971No Hit
CGACACAGACAGTACGTACTCTGCGTTGATACC260.1014396629081971No Hit
ACGCAGAGTGAATGGGACACAGACAGTACGAAA260.1014396629081971No Hit
AACGCAGAGTGAATGGGACACAGAAAAAAAAAA260.1014396629081971No Hit
TATCTACGCAGAGTGAATGGGACACAGACAGAA260.1014396629081971No Hit
TATCAACGCAGAGTGAATGGGGACACAGACAGA260.1014396629081971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT356.258364E-627.01
TATCATC356.258364E-627.02
CAACTCA504.7530193E-926.9999981
AACTCAG653.0213414E-922.8461532
TCAGAGT707.1104296E-921.2142875
CTCAGAG707.1104296E-921.2142874
ATCATCG454.3807402E-521.03
TCATCGC454.3807402E-521.04
CATCGCA454.3807402E-521.05
ACTCAGA751.5741534E-819.83
TCGCAGA509.853271E-518.97
ATCGCAG509.853271E-518.96
GTATCAA1251.382432E-915.121
GATACCA1201.34823495E-814.62527
TGATACC1201.34823495E-814.62526
TATCAAC2150.014.441861
GTTGATA1252.3090251E-814.0424
TTGATAC1252.3090251E-814.0425
ATACCAC600.00728893213.523
ATCAACG2500.011.882
Produced by FastQC (version 0.11.2)