FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_73.3520000005bbaa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_73.3520000005bbaa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10984
Sequences flagged as poor quality0
Sequence length33
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACGAACCAAGACGTACTCTGCGTTGATACCA840.764748725418791No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA630.5735615440640932No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA580.528040786598689No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA550.5007283321194465No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA530.48252002913328473No Hit
GTGAATGGGCAACGAACCAAAAAAAAAAAAAAA460.41879096868171883No Hit
GTGAATGGGCAACGAACAAAAAAAAAAAAAAAA410.37327021121631465No Hit
ACGCAGAGTGAATGGGCAACGAACCAAAAAAAA360.3277494537509104No Hit
ACGCAGAGTGAATGGGCAACGAACAAAAAAAAA360.3277494537509104No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA340.3095411507647487No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA320.29133284777858703No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT320.29133284777858703No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA320.29133284777858703No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA300.2731245447924253No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA300.2731245447924253No Hit
TATCAACGCAGAGTGAATGGGCAACGAACAAAA300.2731245447924253No Hit
AACCAAGACAAAAAAAAAAAAAAAAAAAAAAAA290.2640203932993445No Hit
AACCAAGACGTACTCAAAAAAAAAAAAAAAAAA290.2640203932993445No Hit
GTGAATGGGACAACGAACCAAAAAAAAAAAAAA280.25491624180626365No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAA260.23670793882010197No Hit
GAGTGAATGGGCAACGAACCAAAAAAAAAAAAA250.22760378732702113No Hit
AACGCAGAGTGAATGGGCAACGAACAAAAAAAA240.21849963583394028No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA230.20939548434085942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.20939548434085942No Hit
AACGCAGAGTGAATGGGCAACGAACCAAAAAAA230.20939548434085942No Hit
CCAACGAACCAAGACGTACTCTGCGTTGATACC210.19118718135469775No Hit
CCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.1820830298616169No Hit
GGATTACCCGCTGAGTTTAAGCATATCAAAAAA190.17297887836853604No Hit
TATCAACGCAGAGTGAATGGGACAACGAACCAA190.17297887836853604No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA180.1638747268754552No Hit
GAGTGAATGGGCAACGAACAAAAAAAAAAAAAA160.14566642388929352No Hit
TATCAACGCAGAGTGAATGGGCAACGAACCAAG160.14566642388929352No Hit
GTATCAACGCAGAGTGAATGGGCAACGAACCAA150.13656227239621266No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA150.13656227239621266No Hit
AACCAAGACGAAAAAAAAAAAAAAAAAAAAAAA150.13656227239621266No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT140.12745812090313183No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC140.12745812090313183No Hit
GCAACGAACCAAGACGTACTCTGCGTTGATACC140.12745812090313183No Hit
GTGAATGGGGCAACGAACCAAAAAAAAAAAAAA140.12745812090313183No Hit
ACGCAGAGTGAATGGGCCAACGAAAAAAAAAAA140.12745812090313183No Hit
ACGCAGAGTGAATGGGCAACGAACCAAGACGTA130.11835396941005098No Hit
AACCAAGACGTACTAAAAAAAAAAAAAAAAAAA130.11835396941005098No Hit
ACGCAGAGTGAATGGGACAACGAACCAAAAAAA130.11835396941005098No Hit
GTGAATGGGCCAACGAACCAAAAAAAAAAAAAA120.10924981791697014No Hit
ATCAACGCAGAGTGAATGGGCAACGAACCAAAA120.10924981791697014No Hit
GAGTGAATGGGACAACGAACCAAAAAAAAAAAA120.10924981791697014No Hit
GAGTGAATGGGCCAACGAACCAAAAAAAAAAAA120.10924981791697014No Hit
GTGAATGGGGCAACGAAAAAAAAAAAAAAAAAA120.10924981791697014No Hit
ACAACGAACCAAGACGTACTCTGCGTTGATACC120.10924981791697014No Hit
GTGAATGGGGCGACCCCAGGTCAGGCGGGATTA120.10924981791697014No Hit
GCAACGAACCAAAAAAAAAAAAAAAAAAAAAAA110.1001456664238893No Hit
AACAAGTACCGCGAGGTAAAAAAAAAAAAAAAA110.1001456664238893No Hit
TATCAACGCAGAGTGAATGGGGCAACGAACCAA110.1001456664238893No Hit
AACCAAGACGTAAAAAAAAAAAAAAAAAAAAAA110.1001456664238893No Hit
CAACGAACCAAAAAAAAAAAAAAAAAAAAAAAA110.1001456664238893No Hit
GGCTAAATACGGGCGAGAGACCGATAGCGAACA110.1001456664238893No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1001456664238893No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC853.4330224E-412.7145181
TCAACGC958.765408E-411.3761473
AATGGGG900.00783798410.5071354
ATCAACG1100.00298088569.8248542
CAACGCA1100.00298088569.8248544
TGAATGG3558.58563E-107.99134252
GTGAATG3601.1314114E-97.88035151
ACGCAGA2007.6688745E-47.43004561
GAATGGG3651.328408E-87.40228753
CGCAGAG2202.498541E-47.36864042
GCAGAGT2200.00211587016.75458673
CAGAGTG2250.0026830026.6044854
AGAGTGA2300.0033820496.46090945
GAGTGAA2700.0027330455.97666176
Produced by FastQC (version 0.11.2)