FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_72.3520000005bb9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_72.3520000005bb9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16267
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCACACTACACACGTACTCTGCGTTGATAC2151.3216942275773036No Hit
CCACACTACACACGTACTCTGCGTTGATACCAC1260.7745742915104199No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA850.5225302760189341No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA790.4856457859470093No Hit
ACTCCACACTACACACGTACTCTGCGTTGATAC780.47949837093502184No Hit
CATCCACACTACACACGTACTCTGCGTTGATAC730.44876129587508456No Hit
GCTCCACACTACACACGTACTCTGCGTTGATAC670.4118768058031598No Hit
CTTCCACACTACACACGTACTCTGCGTTGATAC660.4057293907911723No Hit
CTCCACACTACACACGTACTCTGCGTTGATACC610.37499231573123504No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA600.3688449007192476No Hit
GTCCACACTACACACGTACTCTGCGTTGATACC600.3688449007192476No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.32581299563533533No Hit
TCTCCACACTACACACGTACTCTGCGTTGATAC520.3196655806233479No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.3196655806233479No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA480.29507592057539805No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA470.2889285055634106No Hit
CGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA440.27048626052744823No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA400.24589660047949838No Hit
GTGTAGTGTGGAAAAAAAAAAAAAAAAAAAAAA400.24589660047949838No Hit
CGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA400.24589660047949838No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.23974918546751092No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA370.227454355443536No Hit
CGTCCACACTACACACGTACTCTGCGTTGATAC350.21515952541956107No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA330.20286469539558616No Hit
GGTGTGTAGTGTGGAAAAAAAAAAAAAAAAAAA330.20286469539558616No Hit
GGTCCACACTACACACGTACTCTGCGTTGATAC320.1967172803835987No Hit
ATCCACACTACACACGTACTCTGCGTTGATACC310.19056986537161125No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT300.1844224503596238No Hit
GTGAATGGGGTGTGTAGTGTGGAAAAAAAAAAA300.1844224503596238No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA300.1844224503596238No Hit
CTACACACGTACTCAAAAAAAAAAAAAAAAAAA290.17827503534763633No Hit
GTTCCACACTACACACGTACTCTGCGTTGATAC290.17827503534763633No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA280.17212762033564888No Hit
ATTCCACACTACACACGTACTCTGCGTTGATAC270.1659802053236614No Hit
GGTGTGTAGTGTGAAAAAAAAAAAAAAAAAAAA270.1659802053236614No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA260.15983279031167394No Hit
GTGTAGTGTGAAAAAAAAAAAAAAAAAAAAAAA250.15368537529968648No Hit
AGTCCACACTACACACGTACTCTGCGTTGATAC250.15368537529968648No Hit
CCCCTCCACACTACACACGTACTCTGCGTTGAT240.14753796028769903No Hit
ACACTACACAAAAAAAAAAAAAAAAAAAAAAAA220.13524313026372412No Hit
AATCCACACTACACACGTACTCTGCGTTGATAC220.13524313026372412No Hit
CCCTCCACACTACACACGTACTCTGCGTTGATA210.12909571525173666No Hit
GTGAATGGGGTGTGTAGTGTGAAAAAAAAAAAA210.12909571525173666No Hit
GTGAATGGGTCCACACTAAAAAAAAAAAAAAAA200.12294830023974919No Hit
GATCCACACTACACACGTACTCTGCGTTGATAC190.11680088522776173No Hit
TTTCCACACTACACACGTACTCTGCGTTGATAC190.11680088522776173No Hit
TATCCACACTACACACGTACTCTGCGTTGATAC180.11065347021577426No Hit
GGTCCACACTACACAAAAAAAAAAAAAAAAAAA180.11065347021577426No Hit
TGTCCACACTACACACGTACTCTGCGTTGATAC170.10450605520378681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC1153.5470293E-1016.51393127
GTTGATA1251.1586963E-915.19281826
CGTTGAT1405.7498255E-913.56501725
TCCAAAA802.1937332E-413.48153814
CCAAAAA853.6652607E-412.68850615
TCTGCGT4200.011.30417927
GCGTTGA1852.2704626E-810.9986624
TATCAAC1000.001436277310.7852321
CTCTGCG4800.09.89115726
TCAACGC1100.00317487689.8047554
ATCAACG1100.00317487689.8047553
AATGGGG2101.6044578E-79.629674
TGCGTTG2152.0630796E-79.46396423
CAACGCA1300.00116905529.3333725
TGAATGG4100.09.2069042
GTGAATG4250.09.1991671
CGCAGAG2707.059498E-98.9876932
CTGCGTT2451.3808858E-78.85878622
ACGCAGA2603.8398866E-88.8148521
ACTCTGC5500.08.63228225
Produced by FastQC (version 0.11.2)