FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_68.3520000005bb16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_68.3520000005bb16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26710
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGGAGACTACGTACTCTGCGTTGATACCA3631.3590415574691126No Hit
GTAGGAGACTACGTACTCTGCGTTGATACCACT3181.190565331336578No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA1680.6289779108947959No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA1670.6252339947585175No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1490.5578435043055036No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA1110.4155746911269188No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA1070.4005990265818046No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA1060.396855110445526No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.3856233620366904No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA900.33695245226506926No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.321976787719955No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA780.2920254586297267No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA770.2882815424934482No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.27330587794833394No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC710.2658180456757769No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA680.2545862972669412No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.2433545488581056No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA590.2208910520404343No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA590.2208910520404343No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA580.21714713590415577No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA540.20217147135904154No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA530.198427555222763No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA470.17596405840509172No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA460.17222014226881316No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.17222014226881316No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA460.17222014226881316No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA450.16847622613253463No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA430.1609883938599775No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA420.15724447772369898No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA390.14601272931486334No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA390.14601272931486334No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA370.13852489704230625No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGACAA330.12354923249719206No Hit
GACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAA320.1198053163609135No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA310.11606140022463497No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA310.11606140022463497No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA300.11231748408835641No Hit
CAACGCAGAGTGAATGGGACGTAGGAGACAAAA280.10482965181579933No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA280.10482965181579933No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA270.10108573567952077No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCACT257.4972125E-427.07924527
ATACCAC350.005303762419.3423226
GTATCAA1151.3195677E-714.0751881
GATACCA600.00715820913.53962227
TGATACC600.00715820913.53962226
TATCAAC1502.5496593E-711.6902271
TGCGTTG2450.011.60539127
CTGCGTT2801.2732926E-1110.15471726
TCTGCGT4450.09.432096527
ACGCAGA4500.08.6927321
CGCAGAG4700.08.3228282
CTCTGCG5050.08.31145226
ATCAACG2009.9487435E-58.0932333
GCAGAGT4351.8189894E-128.0622243
CAGAGTG4850.07.7873034
AGAGTGA5150.07.5955915
AGTGAAT5950.07.48113977
ACTCTGC5450.07.45300325
AACGCAG2902.238301E-67.44205431
GAGTGAA5650.07.16215326
Produced by FastQC (version 0.11.2)