FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_65.3520000005bab7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_65.3520000005bab7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2041
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA231.1268985791278785No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA160.7839294463498285No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT140.6859382655560999No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA120.5879470847623713No Hit
CATACGCACGTTACGTACTCTGCGTTGATACCA120.5879470847623713No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA120.5879470847623713No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC110.5389514943655072No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA110.5389514943655072No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA80.39196472317491426No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC80.39196472317491426No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA60.29397354238118567No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC60.29397354238118567No Hit
GTGAATGGGACATACGCACGAAAAAAAAAAAAA60.29397354238118567No Hit
CATACGCACAAAAAAAAAAAAAAAAAAAAAAAA60.29397354238118567No Hit
GAGTTTAAGCATATCAAAAAAAAAAAAAAAAAA50.2449779519843214No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA50.2449779519843214No Hit
GCCATACGCACAAAAAAAAAAAAAAAAAAAAAA50.2449779519843214No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2449779519843214No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC50.2449779519843214No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT40.19598236158745713No Hit
GCCATACGCACGTTACGTACTCTGCGTTGATAC40.19598236158745713No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA40.19598236158745713No Hit
ACCATACGCACGTTACGTACTCTGCGTTGATAC40.19598236158745713No Hit
GAGTGAATGGGCATACGCAAAAAAAAAAAAAAA40.19598236158745713No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA40.19598236158745713No Hit
ACGCAGAGTGAATGGGCATACGCAAAAAAAAAA40.19598236158745713No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19598236158745713No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC40.19598236158745713No Hit
AGGCTGTTCCGAAAAAAAAAAAAAAAAAAAAAA30.14698677119059284No Hit
GTGAATGGGACACCATACGCACGAAAAAAAAAA30.14698677119059284No Hit
CTCCCATACGCACGTTACGTACTCTGCGTTGAT30.14698677119059284No Hit
GGCATACGCAAAAAAAAAAAAAAAAAAAAAAAA30.14698677119059284No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14698677119059284No Hit
GCATACGCACAAAAAAAAAAAAAAAAAAAAAAA30.14698677119059284No Hit
GTGAATGGGGCATACGCACAAAAAAAAAAAAAA30.14698677119059284No Hit
GTTCAGACGTGTGCTCAAAAAAAAAAAAAAAAA30.14698677119059284No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC30.14698677119059284No Hit
ACGCACGTTACGTACTCTGCGTTGATACCACTG30.14698677119059284No Hit
GTTCAGACGTGTGCTCTTCAAAAAAAAAAAAAA30.14698677119059284No Hit
CCCCCCATACGCACGTTACGTACTCTGCGTTGA30.14698677119059284No Hit
GTCATACGCAAAAAAAAAAAAAAAAAAAAAAAA30.14698677119059284No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)