FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_64.3520000005ba9e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_64.3520000005ba9e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22748
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA3881.7056444522595395No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA2351.0330578512396695No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC1560.6857745735888869No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA1460.6418146650254968No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA1250.5494988570423773No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA1060.465975030771936No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC1050.46157903991559696No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC1040.45718304905925794No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC980.43080710392122384No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA940.4132231404958678No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA900.3956391770705117No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA860.3780552136451556No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA850.37365922278881664No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC770.33849129593810445No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA760.33409530508176544No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA740.32530332336908735No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA730.3209073325127484No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA700.30771935994373134No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA690.30332336908739227No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA690.30332336908739227No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC680.2989273782310533No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.2989273782310533No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA680.2989273782310533No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.29453138737471424No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC650.2857394056620362No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC620.27255143309301916No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC620.27255143309301916No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA590.2593634605240021No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA540.237383506242307No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA540.237383506242307No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA530.232987515385968No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC530.232987515385968No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC520.22859152452962897No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.22859152452962897No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC490.21540355196061192No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA480.21100756110427288No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA480.21100756110427288No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA460.20221557939159485No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA450.19781958853525586No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC450.19781958853525586No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT440.19342359767891684No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA430.1890276068225778No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA410.18023562510989977No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC410.18023562510989977No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA410.18023562510989977No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA380.16704765254088272No Hit
AAGCTATGGTGTTCACGTACTCTGCGTTGATAC380.16704765254088272No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA370.16265166168454367No Hit
GCTATGGTGTTCACGTACTCAAAAAAAAAAAAA370.16265166168454367No Hit
TATCAACGCAGAGTGAATGGGCTATGGAAAAAA350.15385967997186567No Hit
AGGCTATGGTGTTCACGTACTCTGCGTTGATAC350.15385967997186567No Hit
ACGCAGAGTGAATGGGCTATGGAAAAAAAAAAA330.14506769825918764No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA330.14506769825918764No Hit
ACGCAGAGTGAATGGGGCTATGGTGAAAAAAAA320.1406717074028486No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC320.1406717074028486No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA300.13187972569017056No Hit
GGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.12748373483383155No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.12748373483383155No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA290.12748373483383155No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA280.12308774397749253No Hit
AACGCAGAGTGAATGGGGCTATGGTGAAAAAAA280.12308774397749253No Hit
TAGCTATGGTGTTCACGTACTCTGCGTTGATAC280.12308774397749253No Hit
GTGAATGGGGCTATGGTAAAAAAAAAAAAAAAA280.12308774397749253No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA270.1186917531211535No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA270.1186917531211535No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA270.1186917531211535No Hit
GTGAATGGGGCTATGGAAAAAAAAAAAAAAAAA270.1186917531211535No Hit
GCTATGGTGTTCACGTACTCTTCGTTGATACCA270.1186917531211535No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGTAA260.11429576226481448No Hit
GGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.11429576226481448No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGTAAAA250.10989977140847547No Hit
GGCTATGGTGTAAAAAAAAAAAAAAAAAAAAAA250.10989977140847547No Hit
CAACGCAGAGTGAATGGGCTATGGTGAAAAAAA250.10989977140847547No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA230.10110778969579742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC1950.018.027
GTTGATA1900.017.76315926
CGTTGAT1950.017.30769325
GTATCAA1750.016.971431
GCGTTGA2200.015.34090924
GATACCA550.004073323714.72727327
TGCGTTG2450.013.77551123
TATCAAC2400.012.93751
TCTGCGT5500.012.27272727
CTGCGTT2850.011.84210522
TCAACGC2800.011.0892863
ATCAACG2850.010.8947362
CTCTGCG6450.010.465116526
CAACGCA3100.010.01612954
ACTCTGC7050.09.57446825
GCGCTAT1150.0046551349.3913047
TACTCTG7550.08.94039724
GTACTCT7900.08.54430423
TGGTGTT7900.08.54430413
CGCTATG3755.0931703E-118.2800018
Produced by FastQC (version 0.11.2)