FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_62.3520000005ba5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_62.3520000005ba5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21187
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAGAGCATACGTACTCTGCGTTGATACCAC2951.3923632416104215No Hit
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA2511.1846887242176807No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA1610.7598999386416199No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA1580.7457403124557511No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1250.5899844244111955No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA1090.5144664180865625No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA1030.4861471657148252No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA1000.47198753952895645No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA840.3964695332043234No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA820.38702978241374425No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA820.38702978241374425No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA750.35399065464671736No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.35399065464671736No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA700.33039127767026955No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA670.3162316514844008No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA640.3020720252985321No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA630.29735214990324255No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA620.292632274507953No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA610.2879123991126634No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA610.2879123991126634No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA600.28319252371737386No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA590.2784726483220843No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2643130221362156No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA560.2643130221362156No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA560.2643130221362156No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA550.25959314674092604No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA540.2548732713456365No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT540.2548732713456365No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA530.2501533959503469No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA490.23127389436918866No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA490.23127389436918866No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC490.23127389436918866No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.2218341435786095No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA470.2218341435786095No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA460.21711426818331997No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA460.21711426818331997No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC410.19351489120687212No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC410.19351489120687212No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT390.18407514041629303No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT390.18407514041629303No Hit
ATGGGCAACAGAGCAAAAAAAAAAAAAAAAAAA370.17463538962571387No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA370.17463538962571387No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA370.17463538962571387No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA370.17463538962571387No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA370.17463538962571387No Hit
GGGCAACAGAGAAAAAAAAAAAAAAAAAAAAAA350.16519563883513477No Hit
ACGCAGAGTGAATGGGGCAACAGAAAAAAAAAA350.16519563883513477No Hit
CAACGCAGAGTGAATGGGCAACAGAGCAAAAAA330.15575588804455562No Hit
CTGCAACAGAGCATACGTACTCTGCGTTGATAC310.1463161372539765No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCAAAA300.14159626185868693No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA300.14159626185868693No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA300.14159626185868693No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA300.14159626185868693No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA290.13687638646339736No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGAAAAA290.13687638646339736No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGAAA280.1321565110681078No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA270.12743663567281824No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA260.12271676027752866No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA260.12271676027752866No Hit
CAACAGAGAAAAAAAAAAAAAAAAAAAAAAAAA250.11799688488223911No Hit
TATCAACGCAGAGTGAATGGGCAACAGAAAAAA250.11799688488223911No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCATAC240.11327700948694955No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA240.11327700948694955No Hit
GAGTGAATGGGCAACAGAGCATAAAAAAAAAAA240.11327700948694955No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA230.10855713409165998No Hit
AACGCAGAGTGAATGGGGCAACAGAGCAAAAAA230.10855713409165998No Hit
GAGCATACAAAAAAAAAAAAAAAAAAAAAAAAA230.10855713409165998No Hit
GGGCAACAGAGCATACGTACTCTGCGTTGATAC230.10855713409165998No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA230.10855713409165998No Hit
CGGCAACAGAGCATACGTACTCTGCGTTGATAC230.10855713409165998No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAT220.10383725869637041No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATG200.00842400527.01
GTATCAT200.00842400527.01
TATCATC200.00842400527.02
ATCATCG200.00842400527.03
TCATCGC200.00842400527.04
CATCGCA257.5752725E-427.05
ATACCAC558.9551395E-619.63636427
TATCAAC1700.019.0588251
ATCAACG1950.015.9230782
TCAACGC2050.014.4878043
GATACCA802.2183312E-413.526
TGATACC802.2183312E-413.525
ACTCAAA1006.8530153E-613.511
TACTCAA956.47534E-512.78947410
CTCAAAA1101.83053E-512.27272712
GTACTCA1001.0466635E-412.1500019
CAACGCA2600.011.9423084
GTTGATA1258.27673E-49.7223
TTGATAC1258.27673E-49.7224
TCAAAAA1453.0352935E-49.31034413
Produced by FastQC (version 0.11.2)