FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_58.3520000005b9d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_58.3520000005b9d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21789
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA3371.5466519803570609No Hit
ATGCAGAGACGTACGTACTCTGCGTTGATACCA2901.3309468080223965No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA1980.9087154068566707No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA1740.7985680848134379No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1480.6792418192666024No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA1280.5874523842305751No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA1070.4910734774427463No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA1020.46812611868373954No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA910.4176419294139245No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT910.4176419294139245No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA840.3855156271513149No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA630.2891367203634862No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.2845472486116848No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.275368305108082No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.275368305108082No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA540.24783147459727387No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA520.23865253109367113No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT500.2294735875900684No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.21570517233466427No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.21111570058286291No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA460.21111570058286291No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC440.20193675707926018No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA400.1835788700720547No Hit
GAGTGAATGGGATGCAGAGACAAAAAAAAAAAA400.1835788700720547No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT390.17898939832025335No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA390.17898939832025335No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA380.17439992656845196No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA380.17439992656845196No Hit
ATGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAA370.1698104548166506No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA360.16522098306484925No Hit
CAACGCAGAGTGAATGGGGAGACGTACGTACTC350.16063151131304787No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.16063151131304787No Hit
GGGGAGACGTACGTACTCAAAAAAAAAAAAAAA350.16063151131304787No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA340.1560420395612465No Hit
GTGAATGGGATGCAGAGAAAAAAAAAAAAAAAA340.1560420395612465No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA330.15145256780944513No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACAA330.15145256780944513No Hit
ACGCAGAGTGAATGGGATGCAGAGACAAAAAAA330.15145256780944513No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA330.15145256780944513No Hit
GAGTGAATGGGATGCAGAGAAAAAAAAAAAAAA320.14686309605764378No Hit
GGGATGCAGAGACAAAAAAAAAAAAAAAAAAAA300.137684152554041No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA300.137684152554041No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA300.137684152554041No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGA290.13309468080223966No Hit
AACGCAGAGTGAATGGGATGCAGAGAAAAAAAA290.13309468080223966No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA290.13309468080223966No Hit
GTGAATGGGAATGCAGAGACAAAAAAAAAAAAA280.1285052090504383No Hit
ACGCAGAGTGAATGGGATGCAGAGACGAAAAAA280.1285052090504383No Hit
ACGCAGAGTGAATGGGATGCAGAGAAAAAAAAA280.1285052090504383No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT270.12391573729863693No Hit
GAGTGAATGGGATGCAGAGACGAAAAAAAAAAA270.12391573729863693No Hit
GTGAATGGGGATGCAGAGACAAAAAAAAAAAAA260.11932626554683556No Hit
GTGAATGGGATGCAGAAAAAAAAAAAAAAAAAA250.1147367937950342No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.1147367937950342No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG240.11014732204323281No Hit
ATGCAGAGACGTACGTACTCTTCGTTGATACCA240.11014732204323281No Hit
GATGCAGAGACAAAAAAAAAAAAAAAAAAAAAA240.11014732204323281No Hit
GTGAATGGGCATGCAGAGACAAAAAAAAAAAAA230.10555785029143146No Hit
GGAATGCAGAGACAAAAAAAAAAAAAAAAAAAA230.10555785029143146No Hit
GTGAATGGGGATGCAGAAAAAAAAAAAAAAAAA220.10096837853963009No Hit
TATCAACGCAGAGTGAATGGGATGCAGAAAAAA220.10096837853963009No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.10096837853963009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA454.3395856E-521.00000227
TGATACC509.7615986E-518.926
GGTATCA651.7755392E-618.6923071
TTGATAC552.0261886E-417.18181825
TGATGCA500.002152891116.1999994
GCGTTGA1852.046363E-911.67567527
TATCAAC1801.762237E-811.2500011
GTATCAA1102.547066E-411.0454552
CTGCGTT2358.543611E-89.1914925
TCTGCGT3601.8189894E-129.00000127
TGCGTTG2401.1816883E-78.99999926
ATCAACG2203.4092063E-68.5909092
TCAACGC2306.211594E-68.2173923
CTCTGCG3951.6370905E-118.20253226
GCAGAGT4301.8189894E-128.162793
CAGAGTG4701.8189894E-127.75531964
ACGCAGA4555.456968E-127.71428631
AGAGTGA4900.07.7142865
CGCAGAG4607.2759576E-127.6304352
ACTCTGC4258.367351E-117.623529425
Produced by FastQC (version 0.11.2)