FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_54.3520000005b94f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_54.3520000005b94f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25569
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTACGATCAGACGTACTCTGCGTTGATACCAC3061.1967617036254838No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1760.6883335288826313No Hit
GATCAGACGTACTCAAAAAAAAAAAAAAAAAAA1650.6453126833274669No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1620.6335797254487857No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1250.48887324494505063No Hit
GTGCTACGATCAGACGTACTCTGCGTTGATACC1170.4575853572685674No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.43020845555164455No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1060.41456451171340297No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT930.36372169423911765No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA910.3558997223199969No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA870.34025577848175526No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA810.31678986272439286No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA760.2972349329265908No Hit
CGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA740.28941296100747No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA730.2855019750479096No Hit
CTGCTACGATCAGACGTACTCTGCGTTGATACC700.27376901716922836No Hit
GTGCTACAAAAAAAAAAAAAAAAAAAAAAAAAA600.23465915757362432No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA590.2307481716140639No Hit
GACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA500.1955492979780203No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.18381634009933906No Hit
GTGAATGGGATCAGACGTACTCAAAAAAAAAAA450.17599436818021824No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA430.16817239626109742No Hit
GAGTACGTCTGATCGTAGCAAAAAAAAAAAAAA420.16426141030153701No Hit
CCCCTGCTACGATCAGACGTACTCTGCGTTGAT410.1603504243419766No Hit
GTGAATGGGTGCTACGAAAAAAAAAAAAAAAAA390.1525284524228558No Hit
TATCAACGCAGAGTGAATGGGATCAGACGTACT380.1486174664632954No Hit
GTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.144706480503735No Hit
TGCTACGATCAGACGTACTCTGCGTTGATACCA330.12906253666549336No Hit
ATGCTACGATCAGACGTACTCTGCGTTGATACC330.12906253666549336No Hit
GTGAATGGGGGCGTGCAAAAAAAAAAAAAAAAA330.12906253666549336No Hit
GACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA320.12515155070593295No Hit
GTCTGATCGTAGCAAAAAAAAAAAAAAAAAAAA320.12515155070593295No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA320.12515155070593295No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT310.12124056474637256No Hit
GGTCTGATCGTAGCAAAAAAAAAAAAAAAAAAA290.11341859282725175No Hit
ACGCAGAGTGAATGGGATCAGACGTACTCAAAA280.10950760686769134No Hit
CCCTTGCTACGATCAGACGTACTCTGCGTTGAT280.10950760686769134No Hit
AATGGGAAGCAGTGGTATCAAAAAAAAAAAAAA280.10950760686769134No Hit
GAGTGAATGGGATCAGACGTACTCAAAAAAAAA270.10559662090813093No Hit
GTGAATGGGGTGCTACAAAAAAAAAAAAAAAAA260.10168563494857054No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATGCT200.00847446526.988266
AATGCTA450.00107350717.9921747
CGTCTGA552.0518419E-417.1743476
ACGTCTG603.985012E-415.7431515
GAGTACG550.00410400814.7208691
GTACGTC550.00410400814.7208693
TGGGTGC657.3199597E-414.532146
TATCAAC2850.013.7308681
GGGTGCT802.2555493E-413.494137
ACTGCTA700.001282252613.4941297
TACGTCT600.00730994613.4941294
TTGATAC956.485873E-512.80897826
ATGGGGC750.002156117512.59452117
ATCAACG3200.012.2290552
TCAACGC3250.012.04091553
TGATACC905.999224E-412.01830127
ATGGGTG800.003498647411.80736355
CTGATCG800.003498647411.80736359
GTTGATA1051.652434E-411.58907525
TCTGATC850.00550218211.1128128
Produced by FastQC (version 0.11.2)