FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_53.3520000005b925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_53.3520000005b925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23108
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG1720.744330967630258No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1610.6967284057469275No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1540.6664358663666263No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1040.45006058507876057No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1020.441405573827246No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA880.38082049506664356No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA810.3505279556863424No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT800.3462004500605851No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA750.32456292193179853No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA720.31158040505452655No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA660.2856153712999827No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA580.2509953262939242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.23801280941665223No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC480.20772027003635105No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC450.1947377531590791No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA410.17742773065604983No Hit
GTGAATGGGGTAGACGAAAAAAAAAAAAAAAAA390.16877271940453523No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT370.1601177081530206No Hit
GTGAATGGGTAGACGTAGCTCAAAAAAAAAAAA370.1601177081530206No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA370.1601177081530206No Hit
ACGCAGAGTGAATGGGTAGACGTAAAAAAAAAA360.15579020252726328No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA340.14713519127574867No Hit
ACGCAGAGTGAATGGGTAGACGTAGAAAAAAAA320.138480180024234No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAAAAA320.138480180024234No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA320.138480180024234No Hit
GTGAATGGGTAGACGTAGCAAAAAAAAAAAAAA310.13415267439847672No Hit
GTGAATGGGGTAGACGTAAAAAAAAAAAAAAAA310.13415267439847672No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.13415267439847672No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.1254976631469621No Hit
ACGCAGAGTGAATGGGGTAGACGAAAAAAAAAA280.12117015752120479No Hit
CTTAGACGTAGCTCACGTACTCTGCGTTGATAC280.12117015752120479No Hit
AACGCAGAGTGAATGGGTAGACGTAGAAAAAAA260.11251514626969014No Hit
GTGAATGGGATAGACGTAAAAAAAAAAAAAAAA260.11251514626969014No Hit
GGTAGACGTAGCTCACGTACTCTGCGTTGATAC250.10818764064393284No Hit
GGGTAGACGTAAAAAAAAAAAAAAAAAAAAAAA250.10818764064393284No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC250.10818764064393284No Hit
CCTTAGACGTAGCTCACGTACTCTGCGTTGATA250.10818764064393284No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGCTC240.10386013501817552No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGAAA240.10386013501817552No Hit
ACGTAGCTCAAAAAAAAAAAAAAAAAAAAAAAA240.10386013501817552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATAC703.5697758E-617.38642727
TATCAAC2250.012.593941
GTTGATA1255.0255367E-611.900043527
CGTTGAT2101.2278178E-910.94700927
ATCAACG2759.094947E-1210.3041322
GCGTTGA2305.518814E-99.99509526
TCAACGC2953.6379788E-119.6055473
TGCGTTG2552.9887815E-89.01518425
TCTGCGT4150.08.79795227
CAACGCA3354.456524E-108.4586154
CTGCGTT2851.8088213E-78.06621724
CTCTGCG4453.6379788E-127.90094826
GCAGAGT4853.6379788E-127.5118493
AATGGGT1800.0026361857.49639274
ACTCTGC4751.4551915E-117.401941325
ACGCAGA5151.8189894E-127.3362751
TACTCTG4802.0008883E-117.32483724
CAGAGTG5059.094947E-127.214354
AACGCAG3753.3724064E-87.1965375
GTACTCT4903.274181E-117.175350723
Produced by FastQC (version 0.11.2)