FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_51.3520000005b8fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_51.3520000005b8fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59178
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA13262.240697556524384No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3810.6438203386393593No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3710.6269221670215283No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC3570.603264726756565No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2150.36331068978336545No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1790.302477271959174No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1670.2821994660177769No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1490.25178275710568115No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1270.21460677954645307No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.2095373280611038No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.2044678765757545No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1140.19263915644327284No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1120.18925952211970665No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1090.18419007063435736No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1050.17743080198722497No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.15377336172226164No Hit
ACTCTGTTCCTCACGTACTCTTCGTTGATACCA830.1402548244279969No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1301159214572983No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA740.12504646997194904No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA680.11490756700125047No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA600.10138902970698571No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.008613843526.9560072
TCTACGC200.008613843526.9560073
TATCTAC200.008613843526.9560071
GATACCA1900.019.99766727
TGATACC1950.019.4849126
GCGTTGA6800.016.76275327
TTGATAC2500.015.18529425
GTTGATA2550.014.88754324
CGTTGAT2850.013.331779523
TATCAAC2750.012.2527311
CAGAGAG850.00570079411.0995329
AGAGAGA850.00570079411.09953210
TCAACGC2950.010.9651563
ATCAACG2950.010.9651562
TGCGTTG10550.010.93305226
CAACGCA3450.010.1573364
ACACTCT2051.4173747E-79.8619547
CTGCGTT11900.09.68449925
CTACTCT2102.0138941E-79.6271464
GTGGTAT2605.0804374E-99.3309261
Produced by FastQC (version 0.11.2)