FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_50.3520000005b8d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_50.3520000005b8d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19629
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1270.6470018849661215No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1210.6164348667787457No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1120.570584339497682No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA870.4432217637169494No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA840.42793825462326146No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA820.4177492485608029No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT820.4177492485608029No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA720.36680421824850984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.3413317030923633No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA610.3107646849049875No Hit
CCCTAGCTCACTACCACGTACTCTGCGTTGATA530.27000866065515305No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA410.20887462428040146No Hit
GTAGTGAGCTAAAAAAAAAAAAAAAAAAAAAAA400.20378012124917214No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA380.19359111518671354No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA330.168118600030567No Hit
CCTTAGCTCACTACCACGTACTCTGCGTTGATA310.1579295939681084No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.14774058790564978No Hit
GTGGTAGTGAGCTAAAAAAAAAAAAAAAAAAAA280.1426460848744205No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT240.12226807274950328No Hit
GTACGTGGTAGTGAGCTAAAAAAAAAAAAAAAA240.12226807274950328No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.11207906668704469No Hit
GAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAA210.10698456365581537No Hit
GGGTGGTAGTGAGCTAAAAAAAAAAAAAAAAAA210.10698456365581537No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC200.10189006062458607No Hit
ACCTAGCTCACTACCACGTACTCTGCGTTGATA200.10189006062458607No Hit
TAGCTCACTACCACGTACTCTGCGTTGATACCA200.10189006062458607No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA200.10189006062458607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA509.703691E-518.927
GCGTTGA1551.8590072E-913.06451627
GTAGCAA853.6839614E-412.70588313
TCTGCGT4850.012.24742327
CGTTGAT800.00343736111.812526
TAGCAAA1204.423753E-511.2514
AGCAAAA1452.6059255E-510.2413815
CTCTGCG6200.09.58064526
GCAAAAA1555.43059E-59.58064516
TGCGTTG2358.32315E-89.19148927
GTATCAA2401.1513657E-79.01
CTGCGTT2751.018816E-88.83636426
ACTCTGC6800.08.73529425
TATCAAC1555.869078E-48.7096771
AATGGGG2203.3423148E-68.5909094
TACTCTG7000.08.48571524
GTACTCT7050.08.42553123
ACGTACT7200.08.25000121
ATCAACG1650.00109328028.1818182
GGTATCA1650.00109328028.1818181
Produced by FastQC (version 0.11.2)