FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_49.3520000005b8b9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_49.3520000005b8b9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21069
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA5652.6816650054582563No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA5282.506051544923822No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA3531.6754473396933884No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA3271.552043286344867No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA3091.4666097109497365No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA3091.4666097109497365No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA2801.3289667283686932No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA2581.2245479139968674No Hit
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA2531.2008163652759978No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA2090.9919787365323461No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.9587545683231287No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1650.7831411077886943No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA1620.7689021785561726No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA1610.7641558688119987No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.745170629835303No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA1560.7404243200911291No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA1540.7309317006027813No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA1540.7309317006027813No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA1530.7261853908586074No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA1510.7166927713702597No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT1240.5885424082775642No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA1190.5648108595566947No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA1080.5126014523707817No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA1010.47937728416156433No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA950.4508994256965209No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA900.42716787697565145No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA820.3891973990222602No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.3844510892780863No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA800.3797047795339124No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA800.3797047795339124No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT800.3797047795339124No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA790.37495846978973846No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC690.3274953723479994No Hit
CAACGCAGAGTGAATGGGCAGACAAAAAAAAAA660.31325644311547773No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA660.31325644311547773No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA650.3085101333713038No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA530.25155441644121695No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.25155441644121695No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA510.24206179695286917No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA500.23731548720869522No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA500.23731548720869522No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA490.23256917746452133No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA480.22782286772034746No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA470.2230765579761735No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA430.2040913189994779No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA420.19934500925530402No Hit
TCAACGCAGAGTGAATGGGCAGACACAAAAAAA420.19934500925530402No Hit
GAATGGGCAGACACAAAAAAAAAAAAAAAAAAA420.19934500925530402No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA410.1945986995111301No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA410.1945986995111301No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.1898523897669562No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA400.1898523897669562No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.18510608002278228No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA380.1803597702786084No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA370.1756134605344345No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA370.1756134605344345No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA370.1756134605344345No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA360.17086715079026057No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA350.16612084104608665No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA340.16137453130191276No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA330.15662822155773887No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA330.15662822155773887No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA320.15188191181356495No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG310.14713560206939105No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA310.14713560206939105No Hit
GGTATCAACGCAGAGTGAATGGGCAGACAAAAA280.13289667283686932No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA270.12815036309269542No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.12815036309269542No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT270.12815036309269542No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.12815036309269542No Hit
TATCAACGCAGAGTGAATGGGCAAAAAAAAAAA260.12340405334852153No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC260.12340405334852153No Hit
ACGCAGAGTGAATGGGCAAAAAAAAAAAAAAAA260.12340405334852153No Hit
AACGCAGAGTGAATGGGCAGACACACGTAAAAA260.12340405334852153No Hit
ACACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.12340405334852153No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.11865774360434761No Hit
AACGTAGAGTGAATGGGCAGACAAAAAAAAAAA250.11865774360434761No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA250.11865774360434761No Hit
ACGCAGAGTGAATGGGATGAGCAGAAAAAAAAA240.11391143386017373No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG240.11391143386017373No Hit
AGACACACGTACTCAAAAAAAAAAAAAAAAAAA220.1044188143718259No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA220.1044188143718259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA200.00844443126.9857141
ATCTACG200.00844443126.9857142
GTATCAT200.00844443126.9857141
TCTACGC200.00844443126.9857143
TATCTAC200.00844443126.9857141
CTACGCA200.00844443126.9857144
GGTATCA404.8224794E-420.2392861
GTTGATA751.5008482E-819.83675427
CGTTGAT803.150126E-818.59695626
TATCAAC2800.018.3117331
GTATCAA1002.2801032E-816.191431
ATCAACG3200.016.0227682
GCGTTGA952.243105E-715.66059625
TCAACGC3450.014.8616983
TGCGTTG1950.013.87185627
CAACGCA3850.013.6680894
AACGCAG4450.011.5219915
CTGCGTT2407.2759576E-1211.27088326
ACGCAGA5800.09.5380546
CGCAGAG6000.09.2201197
Produced by FastQC (version 0.11.2)