FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_46.3520000005b85d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_46.3520000005b85d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115478
Sequences flagged as poor quality0
Sequence length33
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC4750.41133376054313375No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA3380.2926964443443773No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.2554599144425778No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA2460.2130275896707598No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1680.1454822563605189No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC1670.1446162905488491No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1440.12469907688044475No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1420.12296714525710525No Hit
CCCCCCCTCCTTGCAGTAACGTACTCTGCGTTG1390.12036924782209599No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1390.12036924782209599No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA1360.11777135038708672No Hit
GGGTACGTTACTGCAAAAAAAAAAAAAAAAAAA1210.10478186321204039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG455.432048E-827.05833627
TGCGTTT257.687176E-427.05833627
CTCTTCG455.432048E-827.05833624
CTTCGTT455.432048E-827.05833626
GTATCAT257.7855575E-426.9995671
ACGCTGA406.001301E-726.9995651
CTCCCCG200.00857945126.9995651
TCCCCGC200.00859765926.987842
CTGAGTG406.0470666E-726.9761284
ACTCTTC501.5067599E-724.35250323
TCTTCGT501.5067599E-724.35250325
CGCTGAG451.682216E-623.9891912
TAACTTA900.023.98919118
GCTGAGT451.682216E-623.9891913
GTAACTT900.023.98919117
AGCTCTC401.8475512E-523.6041135
TCGCCCT352.0357393E-423.1223964
ATACCAC806.730261E-1121.98489827
AACTTAC1000.021.59027319
AGTAACT1050.020.56216616
Produced by FastQC (version 0.11.2)