FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_45.3520000005b833.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_45.3520000005b833.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83129
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2280.2742725162097463No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2210.2658518687822541No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2170.26104007025225856No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1780.21412503458480195No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1650.1984866893623164No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1480.1780365456098353No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1410.1696158981823431No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1160.1395421573698709No Hit
GTGAATGGGTAGCGTCAAAAAAAAAAAAAAAAA1140.13713625810487315No Hit
GTGAATGGGGTAGCGTCAAAAAAAAAAAAAAAA1030.12390381214738538No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1010.12149791288238762No Hit
GTGAATGGGATAGCGTCAAAAAAAAAAAAAAAA910.10946841655739874No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA850.10225071876240543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAT307.105907E-526.98554414
AGCGTCT307.105907E-526.98554410
TAGATAC307.105907E-526.98554416
ATAAGAT200.00858763226.985543
TGCGTTT401.8005165E-523.66938427
TCGCAGA401.8345181E-523.6123497
ATCGCAG401.8345181E-523.6123496
TCATCGC401.8345181E-523.6123494
CTCAGAG352.024279E-423.1304654
GCGTCTA352.024279E-423.13046511
CGTCTAG352.024279E-423.13046512
ATCATCG454.5556888E-520.9887543
CATCGCA454.5556888E-520.9887545
CAACTCA404.991654E-420.2391551
GTCTAGA404.991654E-420.23915513
CTAGATA450.00110242717.99036215
GTATCAT552.1251837E-417.1726191
AACCTAG500.002232015816.1913245
AACTCAG500.002232015816.1913242
TATCATC604.1260492E-415.7415672
Produced by FastQC (version 0.11.2)