FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_44.3520000005b819.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_44.3520000005b819.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80429
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3010.3742431212622313No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA2520.3133198224521006No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA2150.26731651518730803No Hit
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA1720.21385321214984643No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA1640.20390655111962105No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1540.19147322483183926No Hit
GGGCAGAGTGGCAAAAAAAAAAAAAAAAAAAAA1140.14173991968071217No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.1342799239080431No Hit
AACGCAGAGTGAATGGGCAGAGTGGCAAAAAAA1060.13179325865048677No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA1050.1305499260217086No Hit
GAATGGGCAGAGTGGCAAAAAAAAAAAAAAAAA1030.12806326076415223No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC1020.12681992813537407No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA990.12308993024903953No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA980.12184659762026134No Hit
GTATCAACGCAGAGTGAATGGGCAGAGTGGCAA930.11562993447637047No Hit
GAGTGAATGGGCAGAGTGGCAAAAAAAAAAAAA900.11189993659003594No Hit
ACGCAGAGTGAATGGGCAGAGTGGAAAAAAAAA860.10692660607492321No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGTG200.00852206827.02684224
GCTGCGT200.00852206827.02684227
TATCATC700.026.9931432
CTGAGTG257.7756465E-426.9931414
TCAACAC200.00857414926.9931414
GAGTTGC200.00857414926.99314112
AGAGTTG200.00857414926.99314111
ATCAACA200.00857414926.9931413
TGAGTGA257.7756465E-426.9931415
GTATCAT700.026.9763241
ATCGCAG701.0913936E-1125.0650626
TCATCGC701.0913936E-1125.0650624
CATCGCA701.0913936E-1125.0650625
TCTACGC401.8295234E-523.6193
ATCATCG801.8189894E-1223.6193
CGCTGAG300.002249748422.4942862
GCTGAGT300.002249748422.4942863
ACGCTGA300.002257923122.480271
CTACGCA504.1672774E-621.5945134
CAACTCA707.792551E-921.1956841
Produced by FastQC (version 0.11.2)