FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_41.3520000005b7ae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_41.3520000005b7ae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74127
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA5670.7649034764660649No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2530.3413061367652812No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA2520.33995710065158447No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2470.33321192008310063No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2400.32376866728722326No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1830.24687360880650774No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1730.2333832476695401No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1710.2306851754421466No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1400.1888650559175469No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA1020.1376016835970699No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.13490361136967638No Hit
GTGAATGGGCCTATCGGAAAAAAAAAAAAAAAA1000.13490361136967638No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC940.12680939468749577No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA870.11736614189161844No Hit
GAGTACGTTCAAAAAAAAAAAAAAAAAAAAAAA770.10387578075465079No Hit
GAGTACGTTCAACCAAAAAAAAAAAAAAAAAAA770.10387578075465079No Hit
GCCTATCGGTTGAACGTACTCTGCGTTGATACC760.10252674464095404No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCGT200.00853840627.01352327
CCCCTTG200.00853840627.0135231
CAGCCCT257.766345E-426.995275
TTGCACC257.766345E-426.995274
GATCGGT257.766345E-426.9952711
TACGCCT200.008566637526.995275
TAATCCT200.008566637526.995275
CCGCCCT454.5344816E-520.9963235
TCTGCGT24250.020.32976527
GCGCCTA404.972505E-420.2464526
TCGTTTG404.972505E-420.24645213
AAACCTA501.0195734E-418.8966886
ACACCCT501.0195734E-418.8966886
GATACCA1051.2914825E-1018.00901427
CGTTTGA450.00109822817.99684714
GTTTGAA450.00109822817.99684715
ACACCTA753.721525E-717.9968476
ATCGTTT450.00109822817.99684712
CCTACCT552.1154196E-417.1788085
GTTCCTA653.9928196E-516.6124736
Produced by FastQC (version 0.11.2)