FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_4.3520000005b301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_4.3520000005b301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116313
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA14351.2337399946695555No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA7480.6430923456535383No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA6560.5639954261346539No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA6420.5519589383817802No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA5990.5149897259979539No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA4670.4015028414708588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4210.3619543817114166No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA3930.3378814062056692No Hit
GTGAATGGGTATCGACGTCTAAAAAAAAAAAAA3120.2682417270640427No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA2860.2458882498087058No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2720.23385176205583208No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA2670.22955301642980577No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA2610.22439452167857418No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA2530.21751652867693208No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA2520.21665677955172682No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA2330.20032154617282677No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA2230.1917240549207741No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA2100.1805473162931057No Hit
GTGAATGGGTATCGACGTCTACGTACTCAAAAA2100.1805473162931057No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA2100.1805473162931057No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA2090.1796875671679004No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCT1980.17023032679064248No Hit
ACGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.16937057766543723No Hit
GTGAATGGGTATCGACGTCTACGTACAAAAAAA1900.16335233378900038No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA1840.1581938390377688No Hit
ACGCAGAGTGAATGGGTATCGACGTCTACGTAC1840.1581938390377688No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA1790.15389509341174246No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1740.14959634778571612No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA1740.14959634778571612No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA1730.14873659866051087No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1640.14099885653366348No Hit
GTGAATGGGTATCGACGTCTACGAAAAAAAAAA1570.1349806126572266No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1550.1332611144068161No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA1510.12982211790599502No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1450.12466362315476344No Hit
GAGTGAATGGGGTATCGACGTCAAAAAAAAAAA1430.1229441249043529No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1350.11606613190271078No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA1340.11520638277750551No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA1310.11262713540188972No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA1280.11004788802627394No Hit
GTGAATGGGTATCGACGTCTACGTAAAAAAAAA1280.11004788802627394No Hit
GTATCGACGTCTACGTACTCAAAAAAAAAAAAA1260.10832838977586341No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGTCA1230.10574914240024762No Hit
AACGCAGAGTGAATGGGTATCGACGTCTACGTA1230.10574914240024762No Hit
GGGTATCGACGTCAAAAAAAAAAAAAAAAAAAA1180.1014503967742213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA653.6379788E-1227.00691
TAACGCA200.00860447926.983635
TCCAGTA200.00860447926.983635
TTAACGC200.00860447926.983634
GTATCAT1300.024.9294451
TATCATC1300.024.9079652
TCTACGC1250.022.6662483
CTACGCA1250.022.6662484
ATCATCG1450.022.3312783
TCGCAGA1400.022.1651217
ATCGCAG1400.022.1651216
AACTCAG806.91216E-1121.9241982
GCTGAGT806.91216E-1121.9241983
TACGCAG1300.021.7944685
ACGCTGA757.2941475E-1021.6055181
CGCTGAG757.366907E-1021.5869032
CTGAGTG757.366907E-1021.5869034
TCAGAGT757.366907E-1021.5869035
CTCAGAG757.366907E-1021.5869034
TCATCGC1500.021.5869034
Produced by FastQC (version 0.11.2)