FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_38.3520000005b741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_38.3520000005b741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73286
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCAACAACGTACT14141.9294271757225117No Hit
ACAACGTACTCTGCGAAAAAAAAAAAAAAAAAA7791.0629588188740005No Hit
GTATCAACGCAGAGTGAATGGGCAACAACGTAC7631.0411265453156129No Hit
GTGAATGGGCAACAACGTACTCAAAAAAAAAAA5680.7750457113227628No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCAAAA5530.7545779548617744No Hit
ACAACGTACTCTGCAAAAAAAAAAAAAAAAAAA4980.6795295145048168No Hit
ATCAACGCAGAGTGAATGGGCAACAACGTACTC4190.5717326638102775No Hit
CAACAACGTACTCAAAAAAAAAAAAAAAAAAAA3790.5171519799143084No Hit
ACAACGTACTCTGAAAAAAAAAAAAAAAAAAAA3590.4898616379663237No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCG3390.4625712960183391No Hit
CAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA3270.4461970908495484No Hit
CAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA3170.4325519198755561No Hit
ACAACGTACTCTGCGTAAAAAAAAAAAAAAAAA3100.42300030019376145No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCAAA3000.40935512921976913No Hit
GAGTGAATGGGCAACAACGTACTCAAAAAAAAA2830.38615833856398224No Hit
ACAACGTACTCAAAAAAAAAAAAAAAAAAAAAA2570.35068089403160224No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA2430.331577654668013No Hit
CTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.32884862047321456No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGAA2340.3192970007914199No Hit
GTTCGGCAACAACGTACTCTGCGTTGATACCAC2260.3083808640122261No Hit
GTGAATGGGCAACAACGTACTCTGAAAAAAAAA2260.3083808640122261No Hit
GTGAATGGGCAACAACGTACTCTAAAAAAAAAA2260.3083808640122261No Hit
GGTATCAACGCAGAGTGAATGGGCAACAACGTA2240.3056518298174276No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGC2230.3042873127200284No Hit
GCAACAACGTACTCAAAAAAAAAAAAAAAAAAA2180.29746472723303224No Hit
CAACAACGTACTCTGAAAAAAAAAAAAAAAAAA2120.2892776246486368No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA2110.2879131075512376No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTAAA2070.2824550391616407No Hit
GTGAATGGGCAACAACGTACTCTGCGAAAAAAA2070.2824550391616407No Hit
GTATCATCGCAGAGTGAATGGGCAACAACGTAC1890.25789373140845456No Hit
CAACAACGTACTCTAAAAAAAAAAAAAAAAAAA1880.2565292143110553No Hit
TATCTACGCAGAGTGAATGGGCAACAACGTACT1850.2524356630188576No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1770.2415195262396638No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1760.24015500914226456No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1730.23606145785006685No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1710.2333324236552684No Hit
CAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1690.23060338946046993No Hit
ACAACGTACTCTAAAAAAAAAAAAAAAAAAAAA1590.21695821848647764No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.21422918429167917No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1520.207406598804683No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.20331304751248533No Hit
ACGTACTCTGCGTAAAAAAAAAAAAAAAAAAAA1490.20331304751248533No Hit
ACGTACTCTGCGAAAAAAAAAAAAAAAAAAAAA1490.20331304751248533No Hit
CAACAACGTACTCTGCGAAAAAAAAAAAAAAAA1370.18693884234369457No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1330.18148077395409765No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCTG1330.18148077395409765No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1320.1801162568566984No Hit
GGCAACAACGTACTCAAAAAAAAAAAAAAAAAA1300.17738722266189996No Hit
GTGAATGGGTGTTCGGCAAAAAAAAAAAAAAAA1230.16783560298010533No Hit
GTGAATGGGCAACAACGTACTCTGCAAAAAAAA1210.16510656878530688No Hit
GAGTGAATGGGCAACAACGTACTCTAAAAAAAA1160.15828398329831073No Hit
ACGCAGAGTGAATGGGCAACAACGTACTCTGCA1100.15009688071391533No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTGA1100.15009688071391533No Hit
CAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA1070.14600332942171765No Hit
GTGAATGGGCAACAACGTACTCTGCGTAAAAAA1050.1432742952269192No Hit
AACGCAGAGTGAATGGGCAACAACGTACTCTAA1050.1432742952269192No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1030.14054526103212073No Hit
CAACGTACTCTGAAAAAAAAAAAAAAAAAAAAA1030.14054526103212073No Hit
GAGTGAATGGGCAACAACGTACTCTGAAAAAAA1000.13645170973992304No Hit
CAACGCAGAGTGAATGGGCAACAACGTACTCAA970.13235815844772536No Hit
AACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA960.13099364135032612No Hit
GGGCAACAACGTACTCAAAAAAAAAAAAAAAAA930.1269000900581284No Hit
ACTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.12417105586332997No Hit
ACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.1214420216685315No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA870.11871298747373304No Hit
CAACAACGTACTCTGCAAAAAAAAAAAAAAAAA870.11871298747373304No Hit
GAGTGAATGGGCAACAACGTACTCTGCGAAAAA860.11734847037633382No Hit
GTGTTCGGCAACAACGTACTCTGCGTTGATACC850.11598395327893458No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA830.11325491908413612No Hit
CTGTTCGGCAACAACGTACTCTGCGTTGATACC830.11325491908413612No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA820.1118904019867369No Hit
CTGCGTAAAAAAAAAAAAAAAAAAAAAAAAAAA800.10916136779193844No Hit
CTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1077968506945392No Hit
CAACAACGTACTCTGCGTAAAAAAAAAAAAAAA760.1037032994023415No Hit
GTGTTCGGCAAAAAAAAAAAAAAAAAAAAAAAA750.10233878230494228No Hit
CTCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA740.10097426520754305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTT200.008596824526.9753766
CTGAGTG257.7989505E-426.9753764
CCAATGT200.008596824526.9753765
ACGCTGA200.008596824526.9753761
TACCCTG200.008596824526.9753764
TGAGTGA200.008596824526.9753765
TATCTAC451.6749327E-623.9781131
CGCTGAG300.002256470522.479482
ATCTACG504.182715E-621.58032
TCTACGC504.182715E-621.58033
TACGCAG504.182715E-621.58035
CTACGCA504.182715E-621.58034
AAATGTT501.02513965E-418.8827636
ATGGGAG450.001103499817.9835855
TCTGCGT17400.017.0458427
GTCTGTT654.017046E-516.6002316
ATTGTTC757.4970085E-616.1852257
TTTGTTC757.4970085E-616.1852257
TATCAAC5050.016.0249771
AATGTTC1151.4342186E-714.074117
Produced by FastQC (version 0.11.2)