FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_37.3520000005b727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_37.3520000005b727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46576
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA7091.5222432153899004No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3450.740724836825833No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA2960.6355204397114395No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA2790.5990209549982823No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA2560.5496392992098935No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC2390.5131398144967365No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA2080.4465819306080384No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA1560.3349364479560289No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA1380.2962899347303332No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.2962899347303332No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA1350.2898488491927173No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA1110.23832016489178975No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA1070.22973205084163517No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.21899690827894194No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC900.1932325661284782No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA860.1846444520783236No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA730.1567330814153212No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT700.15029199587770525No Hit
GTGAATGGGGTCGGTACAAAAAAAAAAAAAAAA610.13096873926485744No Hit
GGTCGGTACAAAAAAAAAAAAAAAAAAAAAAAA600.1288217107523188No Hit
GTGAATGGGTACTAGAGACGTACTCAAAAAAAA590.12667468223978012No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA560.12023359670216421No Hit
GTGAATGGGATCGGTACAAAAAAAAAAAAAAAA560.12023359670216421No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT530.11379251116454828No Hit
ACGCAGAGTGAATGGGTCGGTACAAAAAAAAAA530.11379251116454828No Hit
GGTCGGTACTAGAGAAAAAAAAAAAAAAAAAAA520.1116454826520096No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA520.1116454826520096No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.1116454826520096No Hit
GAGTGAATGGGATCGGTACAAAAAAAAAAAAAA510.10949845413947097No Hit
TATCAACGCAGAGTGAATGGGTCGGTACAAAAA500.10735142562693233No Hit
GAGTGAATGGGGTCGGTACAAAAAAAAAAAAAA480.10305736860185502No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG200.00852707427.02
GTATCAT405.871643E-727.01
TCTACGC200.00852707427.03
TATCTAC200.00852707427.01
TATCATC405.871643E-727.02
CGGCTCG200.00852707427.05
ATACCAC401.8045068E-523.62527
TCGCAGA559.34546E-619.6363647
ATCGCAG559.34546E-619.6363646
TCATCGC559.34546E-619.6363644
TCTGCGT10800.019.62527
AAATCGG350.005454970519.2857136
GATACCA501.0078294E-418.926
TGATACC501.0078294E-418.925
CAACTCA450.001088577818.01
CATCGCA601.9788471E-518.05
GTATCAA2300.017.021741
ACCTCGG653.9354123E-516.6153856
ATCATCG653.9354123E-516.6153853
AACTCAG500.002204238416.1999992
Produced by FastQC (version 0.11.2)