FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l01n02_kb_plate1_35.3520000005b6ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l01n02_kb_plate1_35.3520000005b6ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242652
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC27001.1127046140151329No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA14350.5913818967080429No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10520.4335426866458962No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.3737863277450835No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.2958970047640242No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA4840.1994626048827127No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA4400.18132964080246608No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA4350.17926907670243805No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.1755600613223876No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA4230.1743237228623708No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA4150.17102682030232597No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT2900.11951271780162537No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA2860.11786426652160295No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC2850.11745215370159735No Hit
CCCCCAGTCCTTCTGTGACGTACTCTGCGTTGA2820.11621581524158053No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA2780.11456736396155813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTG604.3655746E-1127.05239127
TGAAAGA200.00860262326.9964872
GTATCAT1250.024.8316361
CTCTTCG601.3023964E-924.79802524
CTTCGTT601.3023964E-924.79802526
TGCGTTT451.6601607E-624.0465727
TCTTCGT653.2887328E-922.89048625
ACGCTGA1250.022.6723631
ATACCAC3050.022.61757527
AAACCAC300.00226391222.4924241
GGCGTAG300.00226391222.4924241
CATGGAG300.002266632622.4877784
TATCATC1400.021.2115272
CGCTGAG1400.020.2473662
CAACTCA801.6698323E-920.2431831
TGAGTGA1300.019.7119085
ATCTACG1057.2759576E-1219.2832052
CTGAGTG1400.019.2752384
TCTACGC1057.2759576E-1219.2752383
TTACTCT501.0152256E-418.93667422
Produced by FastQC (version 0.11.2)